中国药物警戒 ›› 2025, Vol. 22 ›› Issue (8): 856-862.
DOI: 10.19803/j.1672-8629.20241036

• 中药抗病毒与解毒机制专栏 • 上一篇    下一篇

双参苓颗粒对镉中毒致小鼠慢性肾功能衰竭模型的影响

谢锐1, 孙绮悦1, 李艳英2, 张敬升1, 赵荣华1, 郭姗姗1, 耿子涵1, 包蕾1, 高双荣1, 崔晓兰1#, 谢丹1, 孙静1*   

  1. 1中国中医科学院中药研究所生物安全实验室,北京 100029;
    2北京盈科瑞创新医药股份有限公司,北京 102200
  • 收稿日期:2024-12-25 出版日期:2025-08-15 发布日期:2025-08-13
  • 通讯作者: *孙静,女,博士,副研究员·硕导,中药抗病毒与抗感染药理。E-mail: jsun@icmm.ac.cn #为共同通信作者。
  • 作者简介:谢锐,女,硕士,中药药理。
  • 基金资助:
    国家自然科学基金项目资助（82104500）; 国家科技重大专项（2024D0528801）; 中国中医科学院科技创新工程项目（CI2021B015）; 国家重点研发计划（2020YFE0205100）

Impacts of Shuangshenling Granules on a Cadmium-Induced Chronic Renal Failure Model of Mice

XIE Rui1, SUN Qiyue1, LI Yanying2, ZHANG Jingsheng1, ZHAO Ronghua1, GUO Shanshan1, GENG Zihan1, BAO Lei1, GAO Shuangrong1, CUI Xiaolan1#, XIE Dan1, SUN Jing1*   

  1. 1Biosafety Laboratory, Institute of Chinese Materia Medica, ChinaAcademy of Chinese Medical Sciences, Beijing 100029, China;
    2Beijing Increase Innovative Drug Co., Ltd., Beijing 102200, China
  • Received:2024-12-25 Online:2025-08-15 Published:2025-08-13

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摘要： 目的 观察双参苓颗粒对镉中毒致小鼠慢性肾功能衰竭模型的影响,为临床应用提供参考。方法 将小鼠按照体质量随机分为正常对照组,模型对照组,肾衰宁片阳性对照组,双参苓颗粒高、中、低（9.0、4.5、2.2 g·kg-1·d-1）3个剂量组,每组20只。采用2 mg·kg-1·d-1无水氯化镉溶液连续腹腔注射30 d（小鼠慢性肾功能衰竭模型）,各给药组造模同时灌胃给药45 d。分别于给药15、45 d眼眶采血,酶联免疫吸附（ELISA）法测定血肌酐（Cr）、血尿素氮（BUN）含量;并收集24 h尿液,测定尿蛋白（TP）含量;实验结束后取小鼠双侧肾脏,苏木精-伊红染色法（HE）检测肾组织病理学改变。结果 与正常对照组比较,模型对照组小鼠给药15、45 d的血清中Cr、BUN含量;尿液中TP含量显著增加（P<0.01）,小鼠24 h尿量在15 d时明显增加,45 d时明显减少（P<0.01）。与模型对照组比较,双参苓颗粒3个剂量组给药15、45 d后,均可显著降低镉中毒小鼠Cr、BUN含量（P<0.01）,改善小鼠肾功能,调节小鼠尿量（P<0.01,P<0.05）;双参苓颗粒3个剂量组给药15 d及高、中剂量组给药45 d后可显著降低TP含量（P<0.01,P<0.05）;双参苓颗粒3个剂量组小鼠对氯化镉引起的肾小球增大、球内系膜细胞增生、肾间质瘀血以及肾曲管肿胀等病理改变,均表现出不同程度的缓解与恢复作用。结论 双参苓颗粒对镉中毒致小鼠慢性肾功能衰竭具有显著的治疗作用。

关键词: 双参苓颗粒, 镉中毒, 慢性肾功能衰竭, 血肌酐, 血尿素氮, 尿蛋白, 小鼠

Abstract: Objective To study the effect of Shuangshenling granules on a chronic renal failure model of mice induced by cadmium poisoning in order to provide data for clinical applications. Methods The mice were randomly divided into six groups based on body mass, with 20 mice in each group: normal control group, model control group, Shenshuaining granule positive control group, and three Shuangshenling granule dosage groups (9.0, 4.5, 2.2 g·kg-1·d-1). Except the normal control group, each group was injected intraperitoneally with 2 mg·kg-1·d-1 anhydrous cadmium chloride solution for 30 days to establish a chronic renal failure model, and received respective drugs via gavage administration for 45 days at the same time. On days 15 and 45, the retro-orbital blood was collected while enzyme-linked immunosorbent assay (ELISA) was used to measure the content of serum creatinine (Cr) and blood urea nitrogen (BUN). The 24-hour urine samples were collected to measure the content of totalprotein (TP). The bilateral kidneys of the mice were excised after the experiment before hematoxylin-eosin (HE) staining was adopted to examine the histopathological changes of renal tissues. Results Compared with the normal control group, the model group showed a significant increase in serum Cr, BUN and TP on days15 and 45 (P<0.01), and the 24-hour urine significantly increased on day 15 but significantly decreased on day 45. Compared with the model control group, the three Shuangshenling granule dosage groups showed significantly decreased contents of serum Cr and BUN (P<0.01), improved renal function, and regulated urine outputs on days 15 and 45 (P<0.01, P<0.05). On day 15, the content of TP in the three Shuangshenling granule dosage groups significantly decreased, and the same effect was observed in Shuangshenling granule high and medium dosage groups on day 45(P<0.01, P<0.05). In the three dosage groups, such histopathological changes induced by cadmium chloride were mitigated as glomerular enlargement, proliferation of glomerular mesangial cells, renal interstitial congestion, and swelling of renal convoluted tubules. Conclusion Shuangshenling granules have a significant therapeutic effect on chronic renal failure induced by cadmium poisoning in mice.

Key words: Shuangshenling Granules, Cadmium Poisoning, Renal Failure Model, Blood Creatinine, Blood Urea Nitrogen, Urinary Protein, Mice

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