中国药物警戒 ›› 2025, Vol. 22 ›› Issue (5): 495-500.
DOI: 10.19803/j.1672-8629.20250043

• 中药抗感染作用机制研究专栏 • 上一篇    下一篇

疏风解毒胶囊调控AIM2炎症小体通路治疗呼吸道合胞病毒感染肺炎研究

包蕾, 耿子涵#, 崔晓兰*   

  1. 中国中医科学院中药研究所,北京 100700
  • 收稿日期:2025-01-17 出版日期:2025-05-15 发布日期:2025-05-19
  • 通讯作者: *崔晓兰,女,博士,研究员,中药抗病毒与抗感染药理研究。E-mail：xlcui@icmm.ac.cn;#为共同通信作者。
  • 作者简介:包蕾,女,硕士,助理研究员,中药抗病毒与抗感染药理研究。
  • 基金资助:
    国家自然科学基金资助项目（82141206）

Therapeutic Effect of Shufeng Jiedu Capsules against Respiratory Syncytial Virus-Infected Pneumonia by Regulating AIM2 Inflammasome Pathway

BAO Lei, GENG Zihan#, CUI Xiaolan*   

  1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2025-01-17 Online:2025-05-15 Published:2025-05-19

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摘要： 目的 研究疏风解毒胶囊抗呼吸道合胞病毒（RSV）可能的作用机制和途径。方法 60只BALB/c小鼠按性别分层、体重区组随机分6组（正常对照组,模型对照组,利巴韦林对照组及疏风解毒胶囊1 872、936、468 mg·kg-16个剂量组）,每组10只（雌雄各半）,组间体重均衡。除正常对照组外,其他组通过滴鼻感染RSV建立肺炎模型。感染后4 h起连续给药4 d,每天灌胃1次。实验第5天处死小鼠,计算肺指数,取肺组织进行RT-PCR检测干扰素诱导的蛋白2（AIM2）、凋亡相关斑点蛋白含有CARD结构域（ASC）、半胱氨酸天冬氨酸蛋白酶 1（Caspase-1）、白介素18（IL-18）、白介素1β（IL-1β）等基因的mRNA表达。同时通过Western Blot分析相应蛋白的表达水平。结果 与正常对照组相比,模型对照组小鼠肺指数显著升高（P<0.01）,表明RSV感染引发了严重的肺部炎症。与模型对照组相比,疏风解毒胶囊各剂量组均能显著降低肺指数,以中、高剂量组的抑制效果尤为明显（P<0.01）。RT-PCR和Western Blot结果显示,疏风解毒胶囊高剂量组显著降低了AIM2、ASC、Pro-Caspase-1、Cleaved-Caspase-1、Pro-IL-18、Cleaved-IL-18、Pro-IL-1β和Cleaved-IL-1β的表达。结论 疏风解毒胶囊通过调控AIM2炎症小体通路可显著抑制RSV感染引发的肺部炎症反应,减轻了肺部组织损伤。

关键词: 疏风解毒胶囊, 呼吸道合胞病毒, 炎症小体信号通路, 肺部炎症, 干扰素诱导的蛋白2

Abstract: Objective To study the possible mechanism and pathway of action through which Shufeng Jiedu capsules combat respiratory syncytial virus (RSV). Methods Sixty BALB/c mice were randomly divided into the normal control group, model control group, ribavirin positive control group, and high, medium and low dose groups of Shufeng Jiedu capsules, with 10 mice in each. All these groups were infected with RSV by nasal drops to establish pneumonia models except the normal control group. Four hours after infection, the drug was administered for four consecutive days, once a day by gavage. On the fifth day of the experiment, the mice were killed, the lung index was calculated, and lung tissues were taken for RT-PCR to detect the mRNA expressions of such genes as absent in melanoma 2 (AIM2), apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), cysteine-aspartic protease 1 (Caspase-1), interleukin 18 (IL-18), and interleukin 1 beta (IL-1β). The expression levels of corresponding proteins were analyzed by Western Blot. Results Compared with the normal control group, the lung indexes of mice in the model control group were significantly increased (P<0.01), suggesting that RSV infection caused severe lung inflammation. Compared with the model control group, the lung indexes in all the other groups of Shufeng Jiedu capsules were significantly reduced, especially in the medium and high dose groups (P<0.01). Results of RT-PCR and Western Blot showed that the expressions of AIM2, ASC, pro-caspase-1, cleaved-caspase-1, pro-IL-18, cleaved-IL-18, pro-IL-1β and cleaved-IL-1β (P<0.05, P<0.01) were significantly reduced in the high dose group. Conclusion Shufeng Jiedu capsules can significantly inhibit lung inflammation caused by RSV infection and mitigate the damage to lung tissues by regulating the AIM2 inflammasome pathway.

Key words: Shufeng Jiedu Capsule, Respiratory Syncytial Virus(RSV), Inflammasome Signaling Pathway, Lung Inflammation, Absent in Melanoma 2（AIM2）

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