中国药物警戒 ›› 2023, Vol. 20 ›› Issue (1): 52-56.
DOI: 10.19803/j.1672-8629.20220289

• 基于感染共性作用机制研究专栏 • 上一篇    下一篇

疏风解毒胶囊联合左氧氟沙星治疗多重耐药铜绿假单胞菌感染性小鼠肺炎的作用研究

孙静, 赵荣华, 包蕾, 郭姗姗, 耿子涵, 李舒冉, 徐英莉, 崔晓兰*   

  1. 中国中医科学院中药研究所,北京100029
  • 收稿日期:2022-05-30 发布日期:2023-01-19
  • 通讯作者: *崔晓兰,女,博士,研究员,中药抗病毒及抗感染药理。E-mail: xlcui@icmm.ac.cn
  • 作者简介:孙静,女,博士,中药药理。
  • 基金资助:
    国家自然科学基金资助项目（82104500）

Effects of Shufeng Jiedu capsules combined with levofloxacin in the treatment of mouse pneumonia infected by multidrug-resistant Pseudomonas aeruginosa

SUN Jing, ZHAO Ronghua, BAO Lei, GUO Shanshan, GENG Zihan, LI Shuran, XU Yingli, CUI Xiaolan*   

  1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100029, China
  • Received:2022-05-30 Published:2023-01-19

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摘要： 目的 明确疏风解毒胶囊与左氧氟沙星联合用药对多重耐药铜绿假单胞菌感染致小鼠肺炎模型及死亡模型的药理作用,为临床应用提供基础研究数据支持。方法 实验动物按体质量随机分为6组,分别为正常对照组、模型对照组、左氧氟沙星单独给药组、疏风解毒胶囊3个剂量（2.08、1.04、0.52 g·kg-1·d-1）组与左氧氟沙星联合给药组。采用多重耐药铜绿假单胞菌滴鼻感染致小鼠肺炎和死亡模型,通过检测小鼠肺指数、肺指数抑制率、肺组织病理变化、肺组织中IL-6和TNF-α含量、小鼠死亡率、平均生存天数、生命延长率等指标综合评价疏风解毒胶囊联合左氧氟沙星对多重耐药铜绿假单胞菌感染性小鼠肺炎的治疗作用。结果 疏风解毒胶囊3个剂量组分别与左氧氟沙星联合给药可在一定程度降低多重耐药铜绿假单胞菌感染小鼠肺指数、减轻小鼠肺组织炎症病变、减少肺组织中IL-6和TNF-α 生成、降低小鼠死亡率,延长小鼠平均存活天数。结论 疏风解毒胶囊与左氧氟沙星联合给药对多重耐药铜绿假单胞菌感染致小鼠肺炎及死亡模型和左氧氟沙星单独给药组比较,具有统计学意义。

关键词: 多重耐药铜绿假单胞菌, 小鼠肺炎, 疏风解毒胶囊, 左氧氟沙星, 联合用药

Abstract: Objective To clarify the pharmacological effect of Shufeng Jiedu capsules combined with levofloxacin on a multidrug-resistant Pseudomonas aeruginosa infection-induced pneumonia model and death model in mice, and provide data for clinical applications. Methods Experimental animals were randomly divided into six groups according to their body weight: the normal control group, model control group, levofloxacin alone group, and combined-administration groups at three doses (2.08, 1.04, 0.52 g-1·kg-1·d-1) of Shufeng Jiedu capsules combined with levofloxacin. Pneumonia and death models in mice were established via intranasal infection with multidrug-resistant Pseudomonas aeruginosa. Then, the lung indexes, inhibition rate of these indexes, pathological changes of lung tissue, contents of IL-6 and TNF-α in lung tissue, mice mortality, average survival, and life extension rates were detected. Results The three-dose groups of Shufeng Jiedu capsules combined with levofloxacin could significantly reduce the lung indexes, mitigate pathological injury to lung tissue, lower the levels of IL-6 and TNF-α production as well as the mortality of mice, and prolong the average survival. Conclusion The combined administration of Shufeng Jiedu capsules and levofloxacin has a significantly better effect on the mice model of pneumonia and death caused by multidrug-resistant Pseudomonas aeruginosa infection compared with the levofloxacin alone group.

Key words: multidrug-resistant Pseudomonas aeruginosa, pneumonia in mice, Shufeng Jiedu capsules, levofloxacin, combined medication

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