中国药物警戒 ›› 2018, Vol. 15 ›› Issue (2): 65-69.

• 基础研究 •    下一篇

淫羊藿不同提取物的小鼠长期毒性研究

王琴1,张盼阳1,袁晓美1,毕亚男1,周昆1,2, 张玥1,2,*   

  1. 1 天津中医药大学中医药研究院，天津 300193;
    2 天津中医药大学方剂学教育部重点实验室，天津 300193
  • 收稿日期:2017-11-28 修回日期:2018-03-28 出版日期:2018-02-20 发布日期:2018-03-28
  • 通讯作者: 张玥，女，博士，实验师，中药药理毒理研究。E-mail：zhangyue@tjutcm.edu.cn
  • 作者简介:王琴，女，在读硕士，中药药理毒理研究。
  • 基金资助:
    国家科技重大专项（2015ZX09501004-003-004）：中药成药性优化技术研究。

Long-term Toxicity of Different Extracts of Epimedium Brevicornu Maxim in Mice

WANG Qin1, ZHANG Pan-yang1, YUAN Xiao-mei1, BI Ya-nan1, ZHOU Kun1,2, ZHANG Yue1,2,*   

  1. 1 Medicine Research Institute, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    2 Key Laboratory of pharmacology of Traditional Chinese Medicine Formulae of Education Ministry, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • Received:2017-11-28 Revised:2018-03-28 Online:2018-02-20 Published:2018-03-28

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摘要： 目的 研究淫羊藿不同提取物小鼠口服给药的长期毒性，为其临床应用提供安全参考依据。方法 144只ICR小鼠，随机分为空白对照组，淫羊藿水提物以及淫羊藿醇提物高、中、低剂量组（80、40、20 g?kg-1生药量，连续灌胃给药8周，给药期间观察小鼠的一般状况，每周称体重1次，末次给药后进行相关血生化指标检测、系统尸检及组织病理学检查。结果 淫羊藿水提物、醇提物连续给药4周后，雌、雄小鼠体重均显著低于对照组（P <0.001），此外，与对照组相比，淫羊藿水提物和醇提物给药组小鼠的脏器系数、血常规指标、血清生化学指标均有一定程度变化（P <0.05）。结论 长时间给予大剂量的淫羊藿提取物对小鼠有一定的毒副作用。

关键词: 淫羊藿水提物, 淫羊藿醇提物, 长期毒性, 毒性研究

Abstract: Objective To study the long-term toxicity of oral administration of different extracts of Epimedium brevicornu Maxim in mice and provide a safety reference for its clinical application. Methods 144 ICR mice were randomly divided into blank control group, high, medium and low dose groups of Epimedium brevicornu Maxim water extract and Epimedium brevicornu Maxim alcohol extract (80, 40, 20 g?kg-1). Continuous gavaging for 8 weeks, the general condition of the mice was observed during the administration, and the body weight was weighed weekly. After the last administration, relevant blood biochemical indexes, system autopsy and histopathological examination were performed. Results Four weeks after continuous administration of Epimedium brevicornu Maxim water extract and ethanol extract, the body weight of female and male mice were significantly lower than the control group(P <0.001). In addition, compared with the control group, the organ coefficient, blood indicators, serum biochemical indicators of Epimedium brevicornu Maxim water extract and ethanol extract groups had a certain degree of change(P <0.05). Conclusion Epimedium brevicornu Maxim extracts at high dose have certain toxic effects on mice for 8 weeks.

Key words: Epimedium brevicornu Maxim water extract, Epimedium brevicornu Maxim alcohol extract, long-term toxicity, toxicity studies

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