中国药物警戒 ›› 2021, Vol. 18 ›› Issue (5): 438-443.
DOI: 10.19803/j.1672-8629.2021.05.08

• 临床常见中药补骨脂、决明子配伍减毒研究专栏 • 上一篇    下一篇

盐补骨脂水提物对不同性别大鼠的长期毒性研究

赵潇1,2, 蔡涛涛2,3, 郭欣1,2, 黄娜娜2, 孙蓉2,4,*   

  1. 1天津中医药大学,天津 301617;
    2山东大学第二医院,山东 济南 250033;
    3潍坊市中医院,山东 潍坊 261000;
    4山东大学高等医学研究院,山东 济南 250012
  • 收稿日期:2020-12-21 出版日期:2021-05-15 发布日期:2021-05-12
  • 通讯作者: *孙蓉,女,教授·博导,中药药理与毒理。E-mail:sunrong107@163.com
  • 作者简介:赵潇,女,硕士,中药药理与毒理。
  • 基金资助:
    国家自然基金面上项目（81773997）;国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）;国家公益性行业科研专项（201507004-03）;泰山学者工程专项经费项目（Ns201511107）

Chronic Toxicity of Salt Psoralea Corylifolia L. Aqueous Extract in Male and Female Rats

ZHAO Xiao1,2, CAI Taotao2,3, GUO Xin1,2, HUANG Nana2, SUN Rong2,4,*   

  1. 1Tianjin University of Traditional Chinese Medicine , Tianjin 301617, China;
    2The Second Hospital, Shandong University, Jinan Shandong 250033, China;
    3Weifang Hospital of Traditional Chinese Medicine, Weifang Shandong 261000, China;
    4Advanced Medical Research Institite, Shandong University, Jinan Shandong 250012, China
  • Received:2020-12-21 Online:2021-05-15 Published:2021-05-12

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摘要： 目的 研究盐补骨脂水提物对不同性别Wistar大鼠的长期毒性反应。方法 将80只正常大鼠随机分为正常组、盐补骨脂低剂量（L）组（0.56 g/kg）、盐补骨脂中剂量（M）组（1.12 g/kg）、盐补骨脂高剂量（H）组（5.6 g/kg），连续给药90 d，恢复期45 d，观察大鼠毒性反应程度、毒性靶器官、作用特点及恢复情况。结果 连续给药90 d后，各药物组大鼠体重、每日摄食量、饮水量与正常组比较无统计学差异（P>0.05）；大鼠血常规、血液生化指标与肝相关的指标变化较为显著，其余指标不同程度升高或降低，恢复期结束几乎恢复至正常水平，无重要的毒理学意义；雌性大鼠与雄性大鼠盐补骨脂H组大鼠胸腺/脑均显著降低（P<0.01），雌性大鼠盐补骨脂H组卵巢/脑显著降低；雄性大鼠附睾/脑显著降低（P<0.01），恢复期结束后恢复正常。盐补骨脂H组、盐补骨脂M组不同性别大鼠肝/脑比值在停药45 d后升高（P<0.05），病理结果显示，盐补骨脂H组雌雄大鼠肝脏发生病理性改变，肝细胞空泡变性。结论 连续90 d给大鼠灌服剂量为5.6 g/kg （相当于临床70 kg人日用量的40倍）盐补骨脂水提物会对大鼠产生明显的毒性，以肝脏损伤为主。

关键词: 盐补骨脂, 水提物, 长期毒性, 大鼠

Abstract: Objective To study the long-term toxicity of salt Psoralea corylifolia L. aqueous extract in male and female Wistar rats. Methods Eighty normal rats were randomly divided into the normal group, low dose (L) group (0.56 g/kg)、medium dose (M) group (1.12 g/kg)、and high dose (H) group (5.6 g/kg). The 90 days of continuous administration were followed by the recovery period of 45 days. The degree of toxic reactions, toxic target organs, action characteristics and rate of recovery were observed. Results After continuous administration of 90 days, there was no significant difference in daily food intake or water intake between each administration group and the normal group (P>0.05). The changes of blood routine indexes, blood biochemical indexes and liver related indexes in rats were more significant, and other indexes increased or decreased to varying degrees, but almost returned to normal at the end of recovery, which was of no toxicological significance. The thymus / brain ratio of female and male rats in salt Psoralea corylifolia L. H group was significantly decreased (P<0.01), so was the ovary / brain ratio of female rats in salt Psoralea corylifolia L. H group and the epididymis / brain ratio of male rats in salt Psoralea corylifolia L. H group (P<0.01), but these ratios returned to normal recovery. The liver / brain ratio of male and female rats in salt Psoralea corylifolia L.H and M group increased 45 days after drug withdrawal (P<0.05). Conclusion Ninety days of continuous administration of 5.6 g/kg (equivalent to 40 times clinical 70 kg daily dosage) of Psoralea corylifolia water extract to rats can cause liver injury.

Key words: salt Psoralea corylifolia L., aqueous extract, chronic toxicity, rats

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