松生拟层孔菌水提物在体外对多巴胺能神经元的保护作用研究

中国药物警戒 ›› 2018, Vol. 15 ›› Issue (10): 582-586.

松生拟层孔菌水提物在体外对多巴胺能神经元的保护作用研究

郭姗姗^1*, Wolf Dieter Rausch²

¹中国中医科学院中药研究所,北京 100029;
²维也纳兽医大学医学生物化学学院,奥地利维也纳 A-1210

收稿日期:2018-09-12 修回日期:2018-11-27 出版日期:2018-10-20 发布日期:2018-11-27
作者简介:郭姗姗,女,博士,副研究员,中药药理学。
基金资助:
中国中医科学院中药研究所自主选题项目（ZXKT17011）：药用真菌桑黄对细胞增殖的抑制作用研究

Study on Neuroprotective Effects of Water Extract of Fomitopsis Pinicola on Dopaminergic Neurons in vitro

GUO Shanshan¹, Wolf Dieter Rausch²

¹中国中医科学院中药研究所，北京 100029;
²维也纳兽医大学医学生物化学学院，奥地利维也纳 A-1210

Received:2018-09-12 Revised:2018-11-27 Online:2018-10-20 Published:2018-11-27

摘要/Abstract

摘要： 目的观察松生拟层孔菌水提物在体外对1-甲基-4-苯基吡啶（1-Methyl-4-phenylpyridine,MPP+）诱导胎鼠中脑多巴胺能细胞元凋亡的保护作用。方法采用酪氨酸羟化酶（tyrosine hydroxylase,TH）染色标记胎鼠中脑多巴胺能神经元,观察MPP+诱导后神经元数量和轴突长度的变化,通过检测还原型烟酰胺腺嘌呤二核苷酸（nicotinamide adenine dinucleotide,NADH）下降率来观察细胞线粒体呼吸链复合酶活性的变化,采用碘化乙啶（propidiumiodide,PI）染色分析细胞凋亡率。结果 MPP+诱导后TH阳性（THir）神经元数量明显减少,轴突长度明显下降,线粒体呼吸链复合酶I活性明显下降,凋亡率明显升高;松生拟层孔菌水提物作用后能明显增加THir神经元数量和轴突长度,其中50、25 μg·mL-1剂量组与MPP+组比较具有显著性差异;其可明显升高线粒体呼吸链复合酶I活性,其中50、25、12.5 μg·mL-1剂量组与MPP+组比较具有显著性差异;其可明显降低细胞凋亡率,其中50、25 μg·mL-1剂量组与MPP+组比较具有显著性差异。结论松生拟层孔菌水提物对MPP+诱导的胎鼠中脑多巴胺能细胞元凋亡具有明显的保护作用。

关键词: 松生拟层孔菌, 多巴胺能神经元, 帕金森, 细胞凋亡, 线粒体呼吸链复合酶I

Abstract: Objective To observe the neuroprotective effects of water extract of Fomitopsis Pinicola on 1-Methyl-4-phenylpyridine (MPP+) induced apoptosis of mesencephala dopaminergic cells in vitro. Methods Mesencephalic dopamin-ergic neurons were labeled by tyrosine hydroxylase(TH) staining to observe the survival of THir neurons and the average length of neurite growth. The activity of mitochondrial respiratory chain complex I was analyzed by descent rate of nicotinamide adenine dinucleotide (NADH). The relative apoptosis rate was analyzed by PI staining. Results The survival of TH immuno-reactive(THir) neurons and the average length of neurite growth increased obviously after MPP+ induction, the activity of mitochondrial respiratory chain complex I decreased remarkably, and the apoptosis rate increased significantly. Compared with the MPP+ control group, the survival of THir neurons and the average length of neurite growth were increased by water extract of Fomitopsis Pinicola at doses of 50 and 25 μg·mL-1 respectively. Water extract of Fomitopsis Pinicola treatment(50, 25 and 12.5 μg·mL-1)dramatically increased the activity of mitochondrial respiratory chain complex I compared with MPP+ control respectively. Compared with the MPP+ control, the relative rates of PI-positive cells were significantly decreased by water extract of Fomitopsis Pinicola at doses of 50 and 25 μg·mL-1 respectively. Conclusion Water extract of Fomitopsis Pinicola showed significant neuroprotective effect on mesencephala dopaminergic cells induced by MPP+.

Key words: Fomitopsis Pinicola, dopaminergic neuron, Parkinson, apoptosis, mitochondrial respiratory chain complex I

中图分类号:

R971

郭姗姗, Wolf Dieter Rausch. 松生拟层孔菌水提物在体外对多巴胺能神经元的保护作用研究[J]. 中国药物警戒, 2018, 15(10): 582-586.

GUO Shanshan, Wolf Dieter Rausch. Study on Neuroprotective Effects of Water Extract of Fomitopsis Pinicola on Dopaminergic Neurons in vitro [J]. Chinese Journal of Pharmacovigilance, 2018, 15(10): 582-586.

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