黄芩苷对宿主因子PACT干扰后流感病毒RNA聚合酶活性的影响

中国药物警戒 ›› 2016, Vol. 13 ›› Issue (3): 129-131.

• 基础与临床研究 • 下一篇

黄芩苷对宿主因子PACT干扰后流感病毒RNA聚合酶活性的影响

郭姗姗,包蕾,崔晓兰

中国中医科学院中药研究所, 北京 100029

收稿日期:2006-01-11 修回日期:2016-04-12 出版日期:2016-03-20 发布日期:2016-04-12
通讯作者: 崔晓兰,女,博士,研究员·博导,中药药理。E-mail: cuixl2812@sina.com
作者简介:郭姗姗,女,博士,副研究员,中药药理。
基金资助:
国家自然科学基金（81303290）;北京市自然科学基金（7132141）;中国博士后基金（2015T80208）;中国中医科学院自主选题项目（ZZ2014041, ZXKT15017,自由探索第二批）。

Effects of Baicalin on Activity of Influenza A Virus RNA Polymerase by Silencing Host Factors PACT

GUO Shan-shan, BAO Lei, CUI Xiao-lan

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100029, China

Received:2006-01-11 Revised:2016-04-12 Online:2016-03-20 Published:2016-04-12

摘要/Abstract

摘要： 目的观察宿主因子PACT屏蔽前后黄芩苷对甲型H1N1流感病毒RNA聚合酶的作用。方法采用双荧光素酶报告基因法检测293T细胞中甲型流感病毒聚合酶的相对表达量,分别判断PACT蛋白屏蔽前后黄芩苷对甲型流感病毒RNA聚合酶活性的影响。结果 PACT沉默前后,黄芩苷对流感病毒RNA聚合酶均呈现明显抑制作用,在PACT沉默后,其作用更加明显。结论沉默PACT蛋白并用黄芩苷处理能明显降低流感病毒RNA聚合酶活性。

关键词: 黄芩苷, 宿主因子PACT, 甲型流感病毒RNA聚合酶

Abstract: Objective To observe effects of baicalin on influenza A virus RNA polymerase before and after silencing the PACT protein. Methods Dual luciferase reporter gene approach was used to detect relative expression of influenza A virus polymerase in 293T cells, and judge the influence of influenza A virus polymerase by treatment with baicalin before and after silencing the PACT protein. Results Baicalin could decrease the relative expression of influenza A virus RNA polymerase, and the effects were more obvious by silencing the host factors PACT. Conclusion Silencing the PACT protein and treating with baicalin can reduce the activity of influenza A virus RNA polymerase.

Key words: baicalin, host factors PACT, influenza A virus RNA polymerase

中图分类号:

R965
R285.5

郭姗姗,包蕾,崔晓兰. 黄芩苷对宿主因子PACT干扰后流感病毒RNA聚合酶活性的影响[J]. 中国药物警戒, 2016, 13(3): 129-131.

GUO Shan-shan, BAO Lei, CUI Xiao-lan. Effects of Baicalin on Activity of Influenza A Virus RNA Polymerase by Silencing Host Factors PACT[J]. Chinese Journal of Pharmacovigilance, 2016, 13(3): 129-131.

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黄芩苷对宿主因子PACT干扰后流感病毒RNA聚合酶活性的影响

Effects of Baicalin on Activity of Influenza A Virus RNA Polymerase by Silencing Host Factors PACT

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