中国药物警戒 ›› 2015, Vol. 12 ›› Issue (6): 325-329.

• 基础及临床研究 • 上一篇    下一篇

菌毒清颗粒体外抗病毒的实验研究

郭姗姗,包蕾,崔晓兰*   

  1. 中国中医科学院中药研究所，北京 100029
  • 收稿日期:2015-05-19 出版日期:2015-06-08 发布日期:2015-07-27
  • 通讯作者: 崔晓兰，女，博士，研究员，博士生导师，中药药理。
  • 作者简介:郭姗姗，女，博士，副研究员，中药药理。

Experimental Study on Antivirus Effects of Junduqing Granule in Vitro

GUO Shan-shan,BAO Lei,CUI Xiao-lan*   

  1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences, Beijing 100029, China
  • Received:2015-05-19 Online:2015-06-08 Published:2015-07-27

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摘要： 目的 观察菌毒清颗粒的体外抗病毒作用。方法 采用细胞病变的CPE法观察菌毒清颗粒在体外对呼吸道合胞病毒、腺病毒、柯萨奇病毒的抑制作用，采用实时荧光定量PCR法观察菌毒清颗粒对EB病毒BcLF和BARF1基因表达的抑制作用。结果 菌毒清颗粒对呼吸道合胞病毒、腺病毒、柯萨奇病毒所致CPE均有明显的抑制作用，对EB病毒的BcLF和BARF1基因表达具有明显的抑制作用。结论 菌毒清颗粒能在体外抑制呼吸道病毒、肠道病毒和疱疹科病毒。

关键词: 菌毒清颗粒, 呼吸道合胞病毒, 腺病毒, 柯萨奇病毒, EB病毒

Abstract: Objective To observe the antivirus effects of Junduqing granule in vitro. Methods The cytopathic effect (CPE) method was adopted to study the inhibitory effect of Junduqing granule on respiratory syncytial virus (RSV), adenovirus (AdV) and coxsackie virus (CoxV) in vitro. The quantitative real-time PCR method was used to observe the inhibiting effect of Junduqing granule on EB virus BcLF and BARF1 genetic expression. Results Junduqing granule showed obvious inhibiting effect on the CPE induced by RSV, AdV and CoxV. And it also has obvious inhibiting effect on EB virus BcLF and BARF1 genetic expression. Conclusion Junduqing granule can inhibit respiratory virus, enterovirus and EB virus in vitro.

Key words: Junduqing granule, respiratory syncytial virus, adenovirus, coxsackie virus, epstein-barr virus

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