中国药物警戒 ›› 2014, Vol. 11 ›› Issue (6): 331-332.

• 质量与工艺研究 • 上一篇    下一篇

GC-MS蒙古苍耳挥发油成分分析

姚慧娟1, 姚慧敏2, 卜书红1, 李莉霞1, 陆晓彤1, 张健1   

  1. 1上海交通大学医学院附属新华医院药剂部,上海 200092;
    2通化师范学院吉林省长白山药用植物研究重点实验室,吉林 通化 134002
  • 收稿日期:2013-11-25 修回日期:2016-02-03 出版日期:2014-06-08 发布日期:2016-03-02
  • 作者简介:姚慧娟,女,硕士,药师,中药新药和药理研究。
  • 基金资助:
    吉林省科技厅青年基金项目（20100140）

Analysis of the Volatile Components of Xanthium Mongolicum Kitag by GC-MS

YAO Hui-juan1, YAO Hui-min2, BU Shu-hong1, LI Li-xia1, LU Xiao-tong1, ZHANG Jian1   

  1. 1 Xinhua Hospital Affiliated to School of Medicine of Shanghai Jiaotong University, Shanghai 200092, China;
    2Tonghua Normal University Jilin Key Laboratory of Changbai Mountain Medicinal Plants Research, Jilin Tonghua 134002, China
  • Received:2013-11-25 Revised:2016-02-03 Online:2014-06-08 Published:2016-03-02

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摘要： 目的对蒙古苍耳挥发油成分进行分析研究。方法采用超声波提取法提取蒙古苍耳中的挥发性成分,GC毛细管柱色谱法进行分离,质谱检测器进行分析,峰面积归一化法确定其相对含量,气相色谱-质谱联用技术辅助人工检索鉴定其化学成分。结果从蒙古苍耳的挥发油中分离鉴定出23种化学成分,主要成分有异硫氰酸甲酯（2.92%）,双（三甲基硅基）雌二醇（1.85%）,维生素E（1.33%）,五味子醇甲（1.78%）,秋酰胺（3.09%）,谷甾醇（1.63%）,4,4-二甲基胆甾（15.54%）,秋水仙碱（1.86%）,3,7,11,15 -四甲基-十六醇（13.34%）,α-亚麻酸（16.02%）,γ-亚麻酸（30.12%）,粪甾烯（2.56%）,碱性艳绿（4.49 %）,豆甾醇（1.09%）,上述14种成分含量占总含量的99.7 %。结论蒙古苍耳挥发性成分中亚麻酸总含量最高,占挥发性成分总量的46.14%。

关键词: 蒙古苍耳, 超声波提取法, 挥发性成分, 气相色谱-质谱法

Abstract: ObjectiveTo analyze the volatile oil constituent of Xanthium mongolicum Kitag. MethodsThe volatile components were extracted by ultrasonic extraction, analyzed by capillary GC. The relative content of chemical components was calculated by peak area normalization method, the chemical components were identified by gas chromatography mass spectrometry(GC-MS). ResultsTwenty-three components were identified, the main identified chemical components account for 99.97% of the total volatile components, including isothiocyanato methane(2.92%), bis(trimethylsilyl)estradiol(1.85%), tocopherol(1.33%), schizandrin(1.78%), hiceinamide(3.09%), sitosterol(1.63%), 4,4-dimethylcholestan(15.54%), colchicin(1.86%), 1-hexadecyn-3-ol, 3,7,11,15-tetramethyl-(13.34%),α-linolenic acid(16.02%), γ-linolenic acid(30.12%), coprostene(2.56%), basic green 1(4.49%), stigmasterol(1.09%).ConclusionThe main chemical component of Xanthium mongolicum Kitag volatile components was linolenic acid, accounting for 46.14% of the total volatile components.

Key words: Xanthium mongolicum Kitag, ultrasonic extraction, volatile component, GC-MS

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