藏药余甘子鞣质部位对HT1080细胞迁移与侵袭能力的影响研究

中国药物警戒 ›› 2014, Vol. 11 ›› Issue (5): 264-267.

藏药余甘子鞣质部位对HT1080细胞迁移与侵袭能力的影响研究

赵海娟^{1, 2}, 龚曼², 常青², 游云^{2*, *}, 张兰珍^{1*, *}

1北京中医药大学中药学院,北京 100029;
2中国中医科学院中药研究所,北京 100700

收稿日期:2016-02-03 修回日期:2016-02-03 出版日期:2014-05-08 发布日期:2016-03-02
作者简介:赵海娟,女,在读硕士,中药药理。
基金资助:
国家自然科学基金资助项目（81274187）; 北京中医药大学自主课题（2009TYB22JS027）

Effect of Tannin Part of Tibetan Medicine Phyllanthus Emblica on the Migration and Invasion of HT1080 Cells

ZHAO Hai-juan^{1, 2}, GONG Man², CHANG Qing², YOU Yun^{2, *}, ZHANG Lan-zhen^{1, *}

Received:2016-02-03 Revised:2016-02-03 Online:2014-05-08 Published:2016-03-02

摘要/Abstract

摘要： 目的探讨藏药余甘子鞣质部位对人纤维肉瘤细胞（HT1080）迁移和侵袭能力的影响。方法采用四甲基偶氮唑盐（MTT）法检测余甘子鞣质部位对HT1080细胞活力的影响。分别采用划痕实验和Transwell侵袭实验法检测余甘子鞣质部位对HT1080细胞迁移和侵袭能力的影响。结果余甘子鞣质部位作用48h能够显著抑制HT1080细胞的增殖,其半数抑制浓度（IC50）为0.174g·L-1。与对照组比较,余甘子鞣质部位质量浓度为0.1g·L-1,作用3h后即可明显降低HT1080细胞迁移面积;在质量浓度为0.025g·L-1时可明显降低HT1080细胞透过胶膜的数量,降低其侵袭能力。结论余甘子鞣质部位可有效抑制人纤维肉瘤细胞迁移和侵袭能力。

关键词: 余甘子, 鞣质部位, 迁移, 侵袭

Abstract: Objective To investigate the effect of tannin part of Tibetan medicine Phyllanthus emblica on the migration and invasion of human fibrosarcoma cells HT1080. Methods MTT assay was performed to detect the inhibitory effect of tannin part of Phyllanthus emblica of different concentrations on proliferation of HT1080 cells. Wound-healing scrape assay and Transwell invasion assay were performed to assess the effect of tannin part on the migration and invasive properties of HT1080 in vitro. Results Tannin part of Phyllanthus emblica significantly inhibited the proliferation of HT1080 cells after 48 hours treatment, the median inhibitory concentration(IC50) was 0.174g·L-1. Compared with the control group, tannin part of emblica group of 0.1g·L-1 concentration significantly reduced the migration areas of HT1080 cells. The number of invasive transmembrane cells was decreased significantly in 0.025g·L-1 tannin part group. Conclusion Tannin part of Phyllanthus emblica could inhibit migration and invasion of human fibrosarcoma cells in vitro.

中图分类号:

R965

赵海娟, 龚曼, 常青, 游云, 张兰珍. 藏药余甘子鞣质部位对HT1080细胞迁移与侵袭能力的影响研究[J]. 中国药物警戒, 2014, 11(5): 264-267.

ZHAO Hai-juan, GONG Man, CHANG Qing, YOU Yun, ZHANG Lan-zhen. Effect of Tannin Part of Tibetan Medicine Phyllanthus Emblica on the Migration and Invasion of HT1080 Cells[J]. Chinese Journal of Pharmacovigilance, 2014, 11(5): 264-267.

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