芍药苷对脑缺血再灌注沙土鼠ATP酶和兴奋性氨基酸的影响

中国药物警戒 ›› 2014, Vol. 11 ›› Issue (12): 717-720.

芍药苷对脑缺血再灌注沙土鼠ATP酶和兴奋性氨基酸的影响

李冬梅, 车薇, 李霞, 曹军平^*

武警总医院,北京 100039

收稿日期:2014-01-06 修回日期:2016-02-03 出版日期:2014-12-08 发布日期:2016-03-02
通讯作者: 曹军平,女,本科,主管药师,医院药学。
作者简介:李冬梅,女,本科,主管药师。
基金资助:
国家自然科学基金（81102870）

Effects of Paeoniflorin on ATPase and EAA in Gerbils with Cerebral Ischemia/Reperfusion Injury

LI Dong-mei, CHE Wei, LI Xia, CAO Jun-ping^*

General Hospital of Armed Police Forces, Beijing 100039, China

Received:2014-01-06 Revised:2016-02-03 Online:2014-12-08 Published:2016-03-02

摘要/Abstract

摘要： 目的探讨芍药苷对沙土鼠脑缺血再灌注（CI/R）损伤的保护作用及其作用机制。方法采用结扎双侧颈总动脉缺血10 min再灌注6 h,建立沙土鼠CI/R模型,随机分为假手术组、模型组、芍药苷3个剂量组（20、10、5 mg·kg-1）,每组10只,术前3天开始腹腔注射给药。观察再灌注6 h内神经症状,计算卒中指数;取脑组织匀浆,定磷法测定ATP酶活性,高效液相色谱法测定谷氨酸（Glu）和天冬氨酸（Asp）含量。结果与模型组比较,芍药苷各剂量均能降低CI/R沙土鼠的卒中指数（P <0.05或P <0.01）,各剂量均可显著升高脑组织Ca2+-ATP酶活性（P <0.05）,高、中剂量组能显著升高Na+-K+-ATP酶活性（P <0.05或P <0.01）,各剂量均能降低脑组织Glu含量（P <0.05或P <0.01）。芍药苷对脑组织Mg2+-ATP酶活性和Asp含量则无明显影响。结论芍药苷预处理对CI/R损伤的保护作用与其保护脑细胞膜ATP酶的活性、抑制Glu的释放、降低兴奋性氨基酸毒性等有关。

关键词: 芍药苷, 脑缺血再灌注损伤, ATP酶, 兴奋性氨基酸

Abstract: ObjectiveTo investigate the effects of paeoniflorin (PAE) on gerbils with cerebral ischemia/reperfusion (CI/R) injury and the mechanisms involved. MethodsGerbil model of CI/R was prepared by bilateral common carotid occlusion for 10 min followed by 6-hour reperfusion. Then gerbils were divided into 5 groups at random (n=10), namely sham, model, PAE high, medium and low dose groups (20, 10 and 5 mg·kg-1, respectively). PAE was injected intraperitoneally(i.p.) for 3 days before the carotid occlusion. Stroke index was calculated during the reperfusion. The ATPase activities in brain tissue homogenate were examined by a phosphate assay and glutamate (Glu) and aspirate (Asp) contents were determined by HPLC. ResultsThe results showed that PAE significantly improved stroke index, compared to the model group (P <0.05 or P <0.01). PAE evidently elevated Ca2+-ATPase activities in brain tissue of CI/R gerbils (P <0.05). The Na+-K+-ATPase activities in PAE high and medium dose groups were significantly higher than the model group (P <0.05 or P <0.01). PAE markedly inhibited the Glu contents in brain tissue of CI/R gerbils (P <0.05 or P <0.01). However, PAE showed no influence on Mg2+-ATPase activities or Asp contents in brain tissue. ConclusionThe effectiveness of PAE pretreatment in CI/R injury appears to be associated with the enhancement of ATPase activities and inhibition of excitatory amino acid (EAA) toxicity in brain tissue.

Key words: paeoniflorin, cerebral ischemia/reperfusion injury, ATPase, excitatory amino acid(EAA)

中图分类号:

R965

李冬梅, 车薇, 李霞, 曹军平. 芍药苷对脑缺血再灌注沙土鼠ATP酶和兴奋性氨基酸的影响[J]. 中国药物警戒, 2014, 11(12): 717-720.

LI Dong-mei, CHE Wei, LI Xia, CAO Jun-ping. Effects of Paeoniflorin on ATPase and EAA in Gerbils with Cerebral Ischemia/Reperfusion Injury[J]. Chinese Journal of Pharmacovigilance, 2014, 11(12): 717-720.

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