中国药物警戒 ›› 2014, Vol. 11 ›› Issue (10): 584-586.

• 基于“回阳救逆”功效的附子效-毒关联评价专栏 • 上一篇    下一篇

附子水提组分对正常大鼠血压影响的“量-时-效”关系研究

李晓宇1, 赵庆华2, 吕莉莉1, 王懿3, 冯群4, 严敏5, 孙蓉1 *   

  1. 1山东省中医药研究院,山东 济南 250014;
    2山东中医药大学,山东 济南 250355;
    3山东省中医院,山东 济南 250011;
    4天津中医药大学,天津 300193,山东 济南250013;
    5山东省疾病预防控制中心,山东 济南250014
  • 收稿日期:2014-09-12 修回日期:2016-02-03 出版日期:2014-10-08 发布日期:2016-03-02
  • 通讯作者: 孙蓉,女,博士,研究员·博导,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:李晓宇,女,研究实习员,中药药理与毒理。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目课题2（2009CB522802）

Experimental Study on "Dose-time- efficacy" Relationship of Blood Pressure Induced by Aconite Water Extract

LI Xiao-yu1, ZHAO Qing-hua2, LV Li-li1, WANG Yi, YAN Min5, SUN Rong1, *   

  1. 1Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine Shandong Jinan 250355, China;
    3Shandong Provincial Hospital of Traditional Chinese Medicine, Shandong Jinan 250011, China;
    4Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    5Shandong Center for Disease Control and Prevention, Shandong Jinan 250014, China
  • Received:2014-09-12 Revised:2016-02-03 Online:2014-10-08 Published:2016-03-02

PDF (PC)

100

摘要： 目的研究药典规定剂量附子水提组分单次给药对正常大鼠血压影响的“量-时-效”关系。方法制备1.35、1.08、0.81、0.54、0.27 g·kg-1（分别相当于药典规定附子用量15、12、9、6、3 g）5个不同剂量组的附子水提组分,给予大鼠灌胃上述剂量的附子水提组分,分别于药前,药后15、30、60、90、120 min,采用BP-100A全自动大小鼠无创血压测量系统观察不同剂量和时间下大鼠血压变化情况,并计算附子水提组分对大鼠正常血压影响的ED50值。结果附子水提组分对大鼠收缩压“量-时-效”关系研究显示：5个剂量组大鼠收缩压在药后30 min达峰,且水提组分1.35 g·kg-1剂量组升高幅度最大,药后120 min,5个剂量组的收缩压均基本回落到给药前水平;附子水提组分对大鼠舒张压“量-时-效”关系研究显示：水提组分对舒张压较给药前开始均有不同程度的升高;附子水提组分对大鼠正常血压影响的ED50值及其95%可信限为0.1474（2.3411~0.3482） g·kg-1。结论附子水提组分在药典规定剂量范围内使大鼠收缩压升高,并呈现一定的“量-时-效”关系,剂量愈高,升压作用愈大,作用时间愈长。

关键词: 附子, 水提组分, 血压, 量-时-效

Abstract: ObjectiveTo study the influences of "dose-time-efficacy" relationship on normal rat blood pressure from a single administration of Aconite water extract in pharmacopoeia dose. MethodsThe 5 different Aconite water extract doses of 1.35, 1.08, 0.81, 0.54, 0.27 g·kg-1 (which correspond in the pharmacopoeia Aconite dose 15, 12, 9, 6, 3 g) were prepared, and rats were given Aconite water extract by gavage respectively. Changes of rat blood pressure according to different time and dose were observed in 15, 30, 60, 90, 120 min respectively by BP-100A automatic non-invasive blood pressure measurement system. And the ED50 of Aconite water extract was tested. ResultsThe "dose-time-efficacy" relationship showed that: compared with normal group, the changes of blood pressure in rat were increasing marks from 15 min to 120 min. The systolic pressure in five dose groups hit the peak in 30 min and Aconite water extract high dose (1.35g·kg-1) is the largest. In 120 min, the systolic pressure in five dose groups were basically back to the front of the gavage. The diastolic blood pressure of rats by Aconite water extract was increasing marks from 15 min to 120 min to some extent. The ED50 and its 95% confidence limit of Aconite water extract were 0.1474 (2.3411~0.3482) g·kg-1. ConclusionAconite water extract in the pharmacopoeia dose range elevates rats systolic blood pressure, and there is a certain relationship of "dose-time-efficacy": higher dose, greater pressor effects and longer action time.

Key words: Aconitum carmichaeli Debx., water extract, blood pressure, dose-time- efficacy

中图分类号: