中国药物警戒 ›› 2012, Vol. 9 ›› Issue (1): 23-25.

• 香加皮毒性研究 • 上一篇    下一篇

香加皮不同组分致小鼠肝毒性与氧化损伤相关性研究

孙蓉1，黄伟1，鲍志烨2，张亚囡2   

  1. 1 山东省中医药研究院，山东济南250014；
    2 山东中医药大学，山东济南250355
  • 收稿日期:2011-08-19 出版日期:2012-01-10 发布日期:2015-08-10
  • 作者简介:孙蓉，女，研究员，硕士生导师，从事中药药理与毒理研究。
  • 基金资助:
    国家973 计划中医基础理论专项课题; 有毒中药的毒性与功效、证候关系的基础研究（课题编号：2009CB522802）

The Research of Oxidative Damage Mechanism in Hepatical Toxical Injury Caused by Different Components Cortex Periplocae from in Mice

SUN Rong1, HUANG Wei1, BAO Zhi-ye2, ZHANG Ya-nan2   

  1. 1 Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2 Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2011-08-19 Online:2012-01-10 Published:2015-08-10

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摘要： 目的观察连续给予香加皮水提组分和醇提组分导致小鼠肝毒性损伤程度与氧化损伤的相关性。方法给小鼠灌胃香加皮水提组分和醇提组分，按含生药量计算香加皮水提组分高、中、低剂量分别为9.0，4.68，1.17 g·kg-1，醇提组分高、中、低剂量组分别为1.25，0.50，0.13 ml·kg-1连续给药7 天，观察小鼠一般状况，检测肝功相关指标，血和肝组织内MDA 含量、SOD 活性、一氧化氮（NO）、一氧化氮合酶（NOS）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）的含量和活性。结果香加皮水提组分和醇提组分均可致血中谷丙转氨酶（ALT）、谷草转氨酶（AST）活性增高，血中和肝组织内MDA 含量增加，同时SOD 活性下降;血和肝组织中一氧化氮（NO）含量增加，一氧化氮合酶（NOS）活性升高;血和肝组织中谷胱甘肽（GSH）含量下降，谷胱甘肽过氧化物酶（GSH-Px）活性下降。上述变化趋势随剂量增加而加重，与空白对照组有明显差异。结论香加皮水提组分和醇提组分多次给药后可导致小鼠肝毒性损伤，其损伤途径与引起机体氧化应激后诱导脂质过氧化有关，且醇提组分的肝毒性损伤程度高于水提组分。

关键词: 香加皮, 不同组分, 肝毒性, 氧化损伤

Abstract: Objective To investigate the problems in outpatient and emergency prescriptions in order to improve prescription quality and promote rational use. Methods58 556 outpatient and emergency prescriptions of our hospital in 2010 were randomly selected. The irrational prescriptions were classified and analyzed in respect of writing and rationality of drug use according to Prescription Management, Guiding principles for clinical application of antibiotics, New Edited Pharmacy, Chinese Clinical Drug Use Principle and Hospital Prescription Evaluation and Management(trial) issued in 2010. ResultsThere were 2 506 irrational prescriptions, accounting for 4.28%. Among the total, nonstandard prescriptions, inaptitude medication prescriptions, abnormal prescriptions were respectively accounted for 9.34%、81.76%、8.90%. ConclusionAfter a series of measures were carried out, the unqualified rate of the second half year gradually decreased with the first half year. The quality of outpatient and emergency prescriptions in our hospital is improving gradually, but further hard work should be carried out to improve prescriptions, quality continuously.

Key words: Cortex Periplocae different components, hepato-toxicity, oxidative damage mechanism