中国药物警戒 ›› 2011, Vol. 8 ›› Issue (2): 65-67.

• 基础及临床研究 •    下一篇

苦参碱抑制人子宫内膜癌细胞增殖转移及机制研究

李军1, 薛晓鸥1, 唐炳华2, 牛建昭2   

  1. 1. 北京中医药大学东直门医院妇科,北京 100700;
    2. 北京中医药大学生物化学教研室,北京 100029
  • 收稿日期:2010-12-17 修回日期:2015-08-27 出版日期:2011-02-10 发布日期:2015-08-27
  • 通讯作者: 薛晓鸥,女,教授,博士生导师,中西医结合妇科肿瘤和内分泌。E-mail： xxo123@sina.com
  • 作者简介:李军,女,副主任医师,中西医结合妇科肿瘤和内分泌。
  • 基金资助:
    2010年度高等学校博士学科点,专项科研基金;课题编号：20100013110011

Effects of Matrine on Proliferation and Metastasis of Human Endometrial Carcinoma Cell Line Ishikawa

LI Jun1, XUE Xiao-ou1, TANG Bing-hua2, NIU Jian-zhao2   

  1. 1. Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China;
    2. Biochemistry Department of Beijing University, Beijing 100029, China
  • Received:2010-12-17 Revised:2015-08-27 Online:2011-02-10 Published:2015-08-27

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摘要： 目的 探讨苦参碱对人子宫内膜癌细胞增殖转移的抑制作用及机制。方法 MTT法检测苦参碱对人子宫内膜癌Ishikawa细胞株的抑制作用;流式细胞术观察对细胞周期的影响;ELISA法检测细胞培养上清液中炎性因子IL-8的浓度;Western印迹法检测经苦参碱干预该细胞48h后,NF- B信号途径中p65的变化。结果 苦参碱可显著抑制Ishikawa细胞生长,并呈时间和剂量依赖性。苦参碱使细胞阻滞于G0/G1期。细胞培养上清液中IL-8的浓度随着干预时间延长而减少（P<0.05）。苦参碱作用48h后,细胞NF- Bp65表达明显下降（P<0.05）。结论 苦参碱具有抑制人子宫内膜癌Ishikawa细胞增殖的作用,使细胞阻滞于G0/G1期;抑制子宫内膜癌转移的作用,可能与抑制IL-8分泌及阻断 NF- B信号通路有关。

关键词: 苦参碱, 人子宫内膜癌Ishikawa细胞株, 细胞周期, IL-8, NF-Bp65

Abstract: Objective To study the effect of Matrine on inflammatory factor IL-8 and NF-кBp65 expression from human endometrial carcinoma cell line Ishikawa. Methods The changes of cell proliferation of Ishikawa cells treated with Matrine were observed by using MTT assay. The cell cycle expressions were determined by flow cytometry.The densities of IL-8 in the supenmatant fluid were measured by ELISA. And NF-кBp65 were determined by western blot assay. Results Matrine could inhibit the proliferation of Ishikawa cells in vitro significantly in a dose and time dependent manner(P<0.05). The G0/G1 phase arrest was induced by treatment after 48 hours(P<0.05). The densities of IL-8 in the supenmatant fluid were decreased. Results revealed that cellular NF-кBp65 levels decreased in Matrine-treated cells at 48 hours(P<0.05). Conclusion Matrine could inhibit Ishikawa cell proliferation, induce G0/G1 phase arrest and by interfering with the NF-кB signaling pathway.

Key words: matrine, human endometrial carcinoma cell liner Ishikawa, cell cycle, IL-8, NF-кBp65

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