中国药物警戒 ›› 2011, Vol. 8 ›› Issue (10): 585-586.

• 基础研究 • 上一篇    下一篇

HPLC法测定东北铁线莲中白头翁素的含量

张海丰1, 孙健2, 滕坤1   

  1. 1 通化师范学院,吉林 通化 134000;
    2 中国中医科学院医学实验中心,北京 100700
  • 收稿日期:2011-07-14 出版日期:2011-10-10 发布日期:2015-07-30
  • 作者简介:张海丰,男,博士,中药化学与新药基础研究。
  • 基金资助:
    白头翁素抗肝癌的物质基础与活性研究,吉教科合字[2009]第268号

Determination of the Contents of Anemonin in Clematis mandshurica Rupr by HPLC

ZHANG Hai-feng1, SUN Jian2, TENG Kun1   

  1. 1 Tonghua Normal University,Jilin Tonghua, 134000, China;
    2 Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2011-07-14 Online:2011-10-10 Published:2015-07-30

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摘要： 目的 建立东北铁线莲中白头翁素高效液相色谱法的测定方法。方法 采用Agilent XDB-C18色谱柱(250mm×4.6mm,5μm),以甲醇-水（40：60）为流动相,流速：0.8mL·min-1,柱温为室温,检测波长为220nm。结果 白头翁素在5.45~109μg·mL-1范围内线性关系良好,r=0.9993,平均加样回收率为102.41%,RSD为2.78%（n=6）。结论 该方法操作简单,灵敏,重现性好,可以用来测定东北铁线莲中白头翁素的含量。

关键词: 东北铁线莲, 高效液相色谱法, 白头翁素

Abstract: Objective To establish a HPLC method for the determination of anemonin in Clematis mandshurica Rupr. Methods Agilent XDB C18(4.6 mm×250 mm, 5μm) column was applied,using. methanol-water(40：60) as mobile phase with the rate of 0.8mL·min-1. The column temperature was room temperature and the detective wavelength was 220 nm. Results A good linearity was obtained over the range of 5.45~109μg·mL-1 for anemonin (r=0.9993). The average recovery(n=6) was 102.41%(RSD=2.78%). Conclusion The method is simple, sensitive,good reproducibility and used as the determination of Anemonin in Clematis mandshurica Rupr.

Key words: Clematis mandshurica Rupr, HPLC, anemonin

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