土藤草颗粒对微晶型尿酸钠致家兔急性痛风性关节炎的药效学研究

doi:10.19803/j.1672-8629.20230619

中国药物警戒 ›› 2024, Vol. 21 ›› Issue (3): 263-268.
DOI: 10.19803/j.1672-8629.20230619

• 基于多模型、多靶点的中药抗感染药效及机制研究专栏（二） • 上一篇下一篇

土藤草颗粒对微晶型尿酸钠致家兔急性痛风性关节炎的药效学研究

姚荣妹^1,2, 毛鑫⁴, 徐英莉¹, 张之斌², 高英杰¹, 包蕾¹, 鲍岩岩¹, 孟凤仙^3,#, 崔晓兰^1,*

¹中国中医科学院中药研究所,北京 100700;
²天津中医药大学生物安全实验室,天津 301617;
³北京中医药大学东方医院风湿病科,北京 100700;
⁴广州白云山星群（药业）股份有限公司,广东广州 510288

收稿日期:2023-10-09 出版日期:2024-03-15 发布日期:2024-03-18
通讯作者: ^*崔晓兰,女,博士,研究员,中药抗病毒及抗感染药理。Email: cuixiaolan2812@126.com;^#为共同通信作者。
作者简介:姚荣妹,女,博士,助理研究员,中药药理学。
基金资助:
国家自然科学基金资助项目（51772032）

Effect of Tutengcao granules on MSU-induced acute gouty arthritis in rabbits

YAO Rongmei^1,2, MAO Xin⁴, XU Yingli¹, ZHANG Zhibin², GAO Yingjie¹, BAO Lei¹, BAO Yanyan¹, MENG Fengxian^3,#, CUI Xiaolan^1,*

¹Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
²Animal Biosafety Level 2 Laboratory of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;
³Department of Rheumatology, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China;
⁴Guangzhou Baiyunshan Xingqun Pharmaceutical Co., Ltd, Guangzhou Guangdong 510288, China

Received:2023-10-09 Online:2024-03-15 Published:2024-03-18

摘要/Abstract

摘要： 目的观察土藤草颗粒对微晶型尿酸钠（monosodium urate,MSU）致家兔急性痛风性关节炎模型的药效学作用。方法选用取雄性日本大耳白兔,随机分为正常对照组,模型对照组,阳性药秋水仙碱组（0.18 mg·kg^-1）,土藤草颗粒高、中、低剂量组分别为600、300、150 mg·kg^-1。通过家兔左后肢膝关节腔内注入MSU的方式建立急性痛风性关节炎模型,给药5天后收集关节液,检测关节腔积液中的白细胞数,测定关节液中炎症细胞因子白介素-6（interleukin-6,IL-6）、白介素-8（interleukin-8,IL-8）、肿瘤坏死因子-α（tumour necrosis factor-α,TNF-α）、白介素-1β（interleukin-1β,IL-1β）、前列腺素E2（prostaglandin E2,PGE2）含量;采血检测血尿酸值,给药前后血清中尿素（urea,UREA）、胆固醇（cholesterol,CHO）、血肌酐（serum creatinine,Cre）、血糖（glucose,GLU）及甘油三酯（triglyceride,TG）含量。结果土藤草颗粒高、中、低剂量组给药5 d后关节腔内白细胞数均有所下降,中剂量组与模型对照组比较有显著性差异（P<0.01）;高、中剂量组有降低血尿酸的趋势;土藤草颗粒对家兔血清中UREA、CHO、Cre、GLU及TG含量均无影响;与模型对照组比较,土藤草颗粒高、中、低剂量组IL-6、IL-8、TNF-α及PGE2含量均明显降低,其中高、中、低剂量组的IL-6、TNF-α 含量,高、中剂量组IL-8含量及中剂量组PGE2含量与之比较有显著性差异（P<0.01）;土藤草颗粒高、中、低剂量组可以一定程度上降低关节液中IL-1β含量。结论土藤草颗粒对MSU致急性关节炎家兔模型有明显的治疗作用,可以减少动物关节腔内白细胞数量、抑制炎症细胞因子的表达,降低血清中尿酸含量。

关键词: 土藤草颗粒, 微晶型尿酸钠, 急性痛风性关节炎, 药效学, 大耳白兔, 白介素-6, 白介素-8, 肿瘤坏死因子

Abstract: Objective To observe the pharmacodynamic effect of Tutengcao granules (TTC) on monosodium urate-induced acute gouty arthritis model in rabbits. Methods Male Japanese big-eared white rabbits were randomly divided into normal control group, model control group, colchicine group (0.18 mg·kg^-1), high (600 mg·kg^-1), medium (300 mg·kg^-1) and (150 mg·kg^-1) low-dose groups. An acute gouty arthritis model was established by injecting monosodium urate (MSU) into the knee joint cavity of the left hind limb of rabbits. Five days after injection, synovial fluid was collected. The number of white blood cells in joint effusion was detected, and the contents of inflammatory cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and prostaglandin E2 (PGE2) in joint fluid were measured. Blood was collected to detect the blood uric acid value, and the serum urea, cholesterolesterol (CHO), serum creatinine (Cre), glucose (GLU) and triglyceride contents (TG) before and after administration. Results The number of white blood cells in the joint cavity of the TTC high, medium and low dose groups all decreased after 5 days of administration, and there was a significant difference between the medium dose group and the model control group (P<0.01). TTC high- and medium-dose groups tended to reduce blood uric acid. TTC had no effect on the contents of UREA, CHO, Cre, GLU and TG in rabbit serum. Compared with the model control group, the contents of IL-6, IL-8, TNF-α and PGE2 in the high, medium and low dose groups of TTC were significantly reduced. Among them, the contents of IL-6 and TNF-α in the high, medium and low dose groups were significantly reduced. There was a significant difference between the IL-8 content in the high-dose group and the medium-dose group and the PGE2 content in the middle-dose group (P<0.01). TTC high, medium and low dose groups can reduce the IL-1β content in synovial fluid to a certain extent. Conclusion TTC has a significant therapeutic effect on the MSU-induced acute arthritis rabbit model. It can reduce the number of white blood cells in the animal’s joint cavity, inhibit the expression of inflammatory cytokines, and reduce the uric acid content in the serum.

Key words: Tutengcao granules, monosodium urate, acute gouty arthritis, pharmacodynamics, big-eared rabbits, interleukin-6, interleukin-8, tumour necrosis factor-α

中图分类号:

姚荣妹, 毛鑫, 徐英莉, 张之斌, 高英杰, 包蕾, 鲍岩岩, 孟凤仙, 崔晓兰. 土藤草颗粒对微晶型尿酸钠致家兔急性痛风性关节炎的药效学研究[J]. 中国药物警戒, 2024, 21(3): 263-268.

YAO Rongmei, MAO Xin, XU Yingli, ZHANG Zhibin, GAO Yingjie, BAO Lei, BAO Yanyan, MENG Fengxian, CUI Xiaolan. Effect of Tutengcao granules on MSU-induced acute gouty arthritis in rabbits[J]. Chinese Journal of Pharmacovigilance, 2024, 21(3): 263-268.

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土藤草颗粒对微晶型尿酸钠致家兔急性痛风性关节炎的药效学研究

Effect of Tutengcao granules on MSU-induced acute gouty arthritis in rabbits

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