中国药物警戒 ›› 2019, Vol. 16 ›› Issue (11): 641-648.
DOI: 10.19803/j.1672-8629.2019.11.01

• 基础与临床研究 •    下一篇

桂枝茯苓丸治疗乳腺增生的网络药理学研究

师伟1, 吕忠民2, 张海鸣2, 聂颖兰2, 孙健2,*   

  1. 1 山东中医药大学附属医院,山东 济南 250355;
    2 中国中医科学院医学实验中心/道地药材国家重点实验室培育基地,北京 100700
  • 收稿日期:2019-11-27 修回日期:2019-11-27 出版日期:2019-11-20 发布日期:2019-11-27
  • 通讯作者: 孙健,男,博士,研究员,中药物质基础及活性机制研究。E-mail：sunjiankw@163.com
  • 作者简介:师伟,男,博士,副主任医师。
  • 基金资助:
    国家自然科学基金资助项目（81102672）：基于耐药转运蛋白的前列腺屏障相关引使药配伍机制研究; 山东省重大科技创新工程项目(2018CXGC1309)：基于多维谱-效联合网络药理学关键技术的桂枝茯苓丸治疗子宫腺肌病的物质基础研究; 中央级公益性科研院所基本科研业务费专项资金(ZZ2016013)：中药复方抗菌作用的网络药理学研究; 山东省泰山学者工程

Network Pharmacological Study on Guizhi Fuling Pills in the Treatment of Mammary Gland Hyperplasia

SHI Wei1, LV Zhongmin2, ZHANG Haiming2, NIE Yinglan2, SUN Jian2,*   

  1. 1 Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan Shandong 250355, China;
    2 State Key Laboratory Breeding Base of Dao-di Herbs, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2019-11-27 Revised:2019-11-27 Online:2019-11-20 Published:2019-11-27

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摘要： 目的 采用网络药理学方法研究桂枝茯苓丸治疗乳腺增生的作用机制。方法 利用中药系统药理学分析平台(TCMSP)、Drugbank数据库和TTD数据库查找乳腺增生的靶标蛋白,取三种方法交集得到的靶蛋白确定为乳腺增生的靶蛋白;运用Uniprot数据库查询靶蛋白对应的基因名称并选择人源蛋白最终得到桂枝茯苓丸调控的乳腺增生靶蛋白;采用TCMSP软件检索复方血栓通的活性成分,并且得到其活性成分对应的靶点;运用Cytoscape3.2.1软件构建桂枝茯苓丸活性成分乳腺增生靶标网络并进行网络拓扑学分析;通过STRING数据库和DAVID数据库对关键靶点进行基因本体(gene ontology,GO)富集分析及基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG) 通路分析。结果 网络药理学研究提示,桂枝茯苓丸可能通过激素类信号转导通路及炎症类信号转导通路,治疗乳腺增生。结论 桂枝茯苓丸治疗乳腺增生具有多成分、多靶点的优点,通过网络药理学方法研究桂枝茯苓丸治疗乳腺增生的活性成分和作用机制,为进一步揭示其作用机制提供了新的思路。

关键词: 桂枝茯苓丸, 乳腺增生, 网络药理学

Abstract: Objective To study the mechanism of Guizhi Fuling pills against mammary glandhyperplasia using network pharmacological methods. Methods TCMSP, Drugbank and TTD databases were searched for the target proteins of mammary gland hyperplasia. The Uniprot database was used to query the corresponding gene names of target proteins and select human proteins to obtain the target proteins of mammary gland hyperplasia regulated by Guizhi Fuling pills. TCMSP software was used to search the target proteins of breast hyperplasia. The active ingredients of Guizhi Fuling pills and their corresponding targets were obtained. Cytoscape 3.2.1 software was used to construct the target network of active ingredients of Guizhi Fuling pills and conduct topological analysis of the network. Gene ontology (GO) enrichment analysis and genome encyclopedia (Kyoto Encyclopedia of Genes and Genomes, KEGG) pathway analysis of key targets were carried out using the STRING database and DAVID database. Results Network pharmacological studies suggested that Guizhi Fuling pills might inhibit breast hyperplasia through inflammatory signaling pathways and hormonal signaling pathways. Guizhi Fuling pills had the multi-component and multi-target advantages in the treatment of mammary gland hyperplasia. Conclusion The active ingredients and mechanism of Guizhi Fuling pills in the treatment of mammary gland hyperplasia have been studied with network pharmacologicalMethods, which can help find out more about its mechanism.

Key words: Guizhi Fuling pills, mammary gland hyperplasia, network pharmacology

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