淫羊藿苷对H2O2诱导HUVECs细胞氧化损伤的保护作用

doi:10.19803/j.1672-8629.2019.10.02

中国药物警戒 ›› 2019, Vol. 16 ›› Issue (10): 581-587.
DOI: 10.19803/j.1672-8629.2019.10.02

淫羊藿苷对H₂O₂诱导HUVECs细胞氧化损伤的保护作用

张荔¹, 黄德红¹, 孙付军^{2, *}, 李贵海²

1 襄阳市中医医院,湖北襄阳 441000;
2 山东省中医药研究院,山东济南 250014

收稿日期:2019-10-24 修回日期:2019-10-24 出版日期:2019-10-20 发布日期:2019-10-24
通讯作者: 孙付军,男,本科,副研究员,中药药理与毒理学。E-mail：sunfujun01@126.com
作者简介:张荔,女,硕士,主管药师,中药药理学。
基金资助:
山东省科技厅科技攻关项目（2011GSF11848）：温阳通脉颗粒保护内皮细胞防治糖尿病血管病变的疗效作用及机理的确定

Protective Effect of Icariin on Oxidative Damage of HUVECs Induced by Hydrogen Peroxide

ZHANG Li¹, HUANG Dehong¹, SUN Fujun^{2, *}, LI Guihai²

1 Xiangyang Hospital of Traditional Chinese Medicine, Hubei Xiangyang 441000, China;
2 Shandong Institute of Traditional Chinese Medicine, Shandong Jinan 250014, China

Received:2019-10-24 Revised:2019-10-24 Online:2019-10-20 Published:2019-10-24

摘要/Abstract

摘要： 目的观察淫羊藿苷（ICA）对H₂O₂诱导的人脐静脉内皮细胞（HUVECs）氧化损伤的保护作用及可能机制。方法 H₂O₂体外诱导HUVECs,构建氧化应激损伤模型,加入不同浓度的淫羊藿苷（12.5 μg·mL^-1, 25 μg·mL^-1, 50 μg·mL^-1）,噻唑蓝（MTT）比色法测定细胞活力,生化法测细胞中超氧化物歧化酶（SOD）活性和上清液中乳酸脱氢酶（LDH）释放量,荧光实时定量PCR（RT-PCR）检测细胞中MCP-1和ICAM-1 mRNA的表达。结果与模型组相比,不同浓度的淫羊藿苷能显著提高受损细胞的细胞活力和SOD活性,减少上清液中LDH释放,下调ICAM-1和MCP-1 mRNA表达,且均具有统计学意义（P <0.05或P <0.01）。结论淫羊藿苷能改善受损细胞氧化指标,降低动脉粥样硬化相关细胞因子表达,改善动脉粥样硬化等血管疾病的进程。

关键词: 淫羊藿苷, HUVECs, H₂O₂, 动脉粥样硬化, MCP-1, ICAM-1

Abstract: Objective To observe the protective effect and its possible mechanism of icariin (ICA) on oxidative damage of human umbilical vein endothelial cells (HUVECs) induced by hydrogen peroxide. Methods An oxidative stress injury model of HUVECs induced by hydrogen peroxide in vitro was established, to which icariin of different concentrations of icariin（12.5 μg·mL^-1, 25 μg·mL^-1, 50 μg·mL^-1) was added in different groups were added. Cell viability was measured by MTT colorimetry, and superoxide dismutase (SOD) activity in cells and lactate dehydrogenase (LDH) released in the supernatant were measured using the biochemical method. Furthermore, MCP-1 and ICAM-1 were detected by real-time fluorescence quantitative PCR (RT-PCR). Results Icariin of different concentrations could significantly increase the cell viability and SOD activity of the damaged cells, and reduce the release of LDH in the supernatant compared with the model group. The difference was statistically significant (P <0.01). Further analysis of the mechanism showed that the expressions of ICAM-1 and MCP-1 mRNA were down-regulated (P <0.01). Conclusion Icariin can improve the oxidation index of damaged cells, reduce the expressions of atherosclerosis-related cytokines, and improve the progression of such vascular diseases such as atherosclerosis.

Key words: icariin, HUVECs, hydrogen peroxide, atherosclerosis, MCP-1, ICAM-1

中图分类号:

R965

张荔, 黄德红, 孙付军, 李贵海. 淫羊藿苷对H₂O₂诱导HUVECs细胞氧化损伤的保护作用[J]. 中国药物警戒, 2019, 16(10): 581-587.

ZHANG Li, HUANG Dehong, SUN Fujun, LI Guihai. Protective Effect of Icariin on Oxidative Damage of HUVECs Induced by Hydrogen Peroxide[J]. Chinese Journal of Pharmacovigilance, 2019, 16(10): 581-587.

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淫羊藿苷对H₂O₂诱导HUVECs细胞氧化损伤的保护作用

Protective Effect of Icariin on Oxidative Damage of HUVECs Induced by Hydrogen Peroxide

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