中国药物警戒 ›› 2011, Vol. 8 ›› Issue (5): 257-259.

• 基础研究 •    下一篇

昆参颗粒对裸鼠人胃癌模型VEGF-C、VEGFR-3表达的影响

赵红1,2, 相宏杰1, 曹志群3   

  1. 1. 山东中医药大学,山东 济南 250014;
    2. 中国人民解放军海军总医院,北京 100048;
    3. 山东中医药大学附属医院,山东 济南 250011
  • 收稿日期:2015-08-27 修回日期:2015-08-27 出版日期:2011-05-10 发布日期:2015-08-27
  • 通讯作者: 曹志群,男,博士,主任医师,消化系统疾病的临床及实验研究。
  • 作者简介:赵红,女,副教授,在读博士研究生,中医消化系统疾病的临床及实验研究。
  • 基金资助:
    国家科技重大专项（2009ZX09502-015）,国家自然科学基金资助项目（30973826）,高校博士点基金项目（20093731110004）,山东省科技厅项目（200221）

Effect of Kunshen Granule on the Expression of VEGF-C、VEGFR-3 of Human Gastric Carcinoma in Nude Mice Model

ZHAO Hong1,2, XIANG Hong-jie1, CAO Zhi-qun3   

  1. 1. Department of Clinical Medicine Shandong University of TCM, Shandong Jinan 250014, China;
    2. PLA Navy general Hospital, Beijing 100048, China;
    3. Affiliated Hospital of Shandong University of TCM, Shandong Jinan 250011, China
  • Received:2015-08-27 Revised:2015-08-27 Online:2011-05-10 Published:2015-08-27

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摘要： 目的 探讨昆参颗粒对裸鼠人胃癌模型血管内皮生长因子C（VEGF-C）、血管内皮生长因子受体-3（VEGFR-3）的影响。方法 将40只BALB/C无胸腺裸鼠随机分为4组：阴性对照组、5-Fu组、昆参高剂量组、昆参低剂量组,每组10只。采用“OB”胶粘贴法建立裸鼠人胃癌SGC-7901原位移植模型,术后48小时开始分别给予生理盐水、5-Fu、昆参高剂量、昆参低剂量治疗,6周后观察裸鼠胃癌淋巴结转移情况,用Envision免疫组化染色法检测原位移植瘤组织中VEGF-C、VEGFR-3蛋白的表达情况。结果 ① 40只裸鼠胃癌原位移植瘤均有生长,部分伴有淋巴结转移,阴性对照组、5-Fu组、昆参高剂量组、昆参低剂量组的淋巴结转移率分别为100%、50%、40%、60%。②药物干预的三组胃癌组织中VEGF-C、VEGFR-3的表达明显少于阴性对照组,有统计学差异（P <0.01）。昆参高剂量组VEGF-C表达明显少于5-Fu组,有统计学差异（P <0.05）。昆参高剂量组比昆参低剂量组VEGF-C、VEGFR-3的表达减少,有统计学差异（P <0.01）。③VEGF-C、VEGFR-3、淋巴结转移之间存在密切直线相关性（r=0.825,P =0.00）。结论 昆参颗粒能明显抑制VEGF-C、VEGFR-3蛋白的表达,可能是昆参颗粒抑制胃癌淋巴结转移的重要作用机制。

关键词: 胃癌, 昆参颗粒, VEGF-C, VEGFR-3, 实验研究

Abstract: Objective To probe the effect of Kunshen Granule on the expression of vascular endothelial growth factor-C(VFGF-C) and vascular endothelial growth factor receptor-3(VFGFR-3). Methods 40 BALB/C nude mice were randomly divided into 4 groups: negative control group, 5-Fu group, Kunshen large dose group and small dose group. The nude mice except the negative group were implanted human cancer cell SGC-7901 on stomach near the greater curvature side by means of plastering "OB" gum. And then nude mice were treated with normal saline, 5-Fu, Kunshen large dose and small dose respectively. After six weeks, the animals were anesthetized, sacrificed and the local growth transplanted tumor were observed. The expressions of VEGF-C and VEGFR-3 in the local transplanted tumor tissues were detected and analyzed semi-quantitatively by means of Envision immunohistochemistry staining. Results ①The transplanted tumors in 40 nude mice were growing well, and some of them had enlarged abdominal lymph nodes, lymph nodes metastasis rates of 5-Fu group, Kunshen large dose group and small dose group were 50%,40%,60%, significantly lower than negative control group, which was 100%.②Compared to negative control group, the expressions of VEGF-C and VEGFR-3 in 5-Fu group, Kunshen large dose group and small dose group were significantly decreased, the difference had a statistical significance(P <0.01). Difference of the expression of VEGF-C between the Kunshen large dose group and 5-Fu group had a statistical significance(P <0.05). Difference of the expression of VEGF-C and VEGFR-3 between the Kunshen large dose group and Kunshen small dose group had a statistical significance(P<0.01).③There is linear correlativity between the expression of VEGF-C, VEGFR-3 and the lymph nodes metastasis nearly(r=0.825, P=0.00). Conclusion Kunshen granule can decreased the expression of the VEGF-C and VEGFR-3, which may be one of the mechanisms inhibiting carcinoma lymph nodes metastasis.

Key words: Kunshen Granule, VEGF-C, VEGFR-3, experimental study

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