中国药物警戒 ›› 2025, Vol. 22 ›› Issue (7): 755-762.
DOI: 10.19803/j.1672-8629.20250213

• 基础与临床研究 • 上一篇    下一篇

前胡属药材中24种香豆素类成分的定量表征及化学模式判别研究

李耀磊1,2, 金红宇1,2,*, 程显隆1,2, 魏锋1,2#   

  1. 1中国食品药品检定研究院中药民族药检定所,北京 102629;
    2药品监管科学全国重点实验室,北京 102629
  • 收稿日期:2025-04-09 出版日期:2025-07-15 发布日期:2025-07-17
  • 通讯作者: *金红宇,男,主任药师,中药质量评价。E-mail：jhyu@nifdc.org.cn #为共同通信作者。
  • 作者简介:李耀磊,男,博士,中药质量评价。
  • 基金资助:
    国家重点研发计划（2022YFC3501505、2023YFC3504100）; 国家自然科学基金资助项目（82404856）

Quantitative Characterization and Chemometric Discrimination of 24 Coumarin Constituents in Peucedanum Medicinal Materials

LI Yaolei1,2, JIN Hongyu1,2,*, CHENG Xianlong1,2, WEI Feng1,2#   

  1. 1Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, China;
    2State Key Laboratory of Drug Regulatory Science, Beijing 102629, China
  • Received:2025-04-09 Online:2025-07-15 Published:2025-07-17

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摘要： 目的 构建前胡属药材中24种香豆素类成分的多组分同步定量分析方法,阐明前胡与紫花前胡的化学特征差异及其种属特异性标志物,为品种鉴定和道地性评价提供参考。方法 基于色谱与质谱联用技术（UPLC-MS/MS）技术建立多组分定量分析方法,系统验证线性范围、灵敏度、精密度、稳定性、基质效应及回收率等,结合化学计量学如主成分分析（PCA）、偏最小二乘法-判别分析（PLS-DA）、聚类分析对多批次前胡及紫花前胡样本进行化学模式解析。结果 基于UPLC-MS/MS法建立的前胡属中24种香豆素类成分分析方法经方法学验证,考察项目均符合分析要求;前胡及紫花前胡成分含量分析显示,两者在香豆素类成分分布上种属特异性和化学特征差异显著,如紫花前胡苷（10~30倍）、7-羟基香豆素（2~10倍）等在前胡中含量远低于紫花前胡;PCA、PLS-DA、聚类分析表明24种香豆素类成分显示前胡及紫花前胡在化学空间中显著分离特点,其中前胡以线型呋喃香豆素中的白花前胡甲素、乙素等为核心标志物,紫花前胡富集角型吡喃香豆素中的异紫花前胡内酯及羟基化衍生物如7-羟基香豆素等。结论 本研究开发的UPLC-MS/MS方法高效、精准,结合化学计量学模型,系统揭示前胡属药材香豆素成分的种属分布规律,为品种鉴定、质量标准化及中药监管提供多指标协同控制策略。

关键词: 白花前胡, 紫花前胡, 白花前胡甲素, 白花前胡乙素, 紫花前胡苷, 香豆素, UPLC-MS/MS, 化学计量学

Abstract: Objective To establish a multi-component simultaneous quantitative analytical method for 24 coumarin constituents in Peucedanum medicinal materials, elucidate the differences in chemical properties and species-specific markers between Peucedanum praeruptorum Dunn (Qianhu) and Peucedanum decursivum (Miq.) Maxim. (Zihua Qianhu) in order to provide a reference for varietal identification and for evaluation of geographical authenticity. Methods Using UPLC-MS/MS technology, a multi-component quantitative analytical method was established, which was validated in terms of linearity, sensitivity, precision, stability, matrix effects, and recovery rates. Chemometric techniques, including principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and hierarchical clustering analysis (HCA), were applied to profile the chemical patterns of multi-batch samples from Peucedanum praeruptorum Dunn and Peucedanum decursivum (Miq.) Maxim. Results The analytical method for 24 coumarins in the genus Peucedanum established based on the UPLC-MS/MS method passed the methodological validation, and all the investigated items met the analytical requirements. The analysis of contents of the components in Peucedanum praeruptorum Dunn and Peucedanum decursivum (Miq.) Maxim. showed that there were significant species-specific and chemical characteristic differences in the distribution of coumarin components between these two plants. The contents of such compounds as nodakenin (10-30 times) and 7-hydroxycoumarin (2-10 times) in Peucedanum praeruptorum Dunn were much lower than those of Peucedanum decursivum (Miq.) Maxim. PCA, PLS-DA, and HCA indicated that the 24 coumarin components were distinctly separated in the chemical space between Peucedanum praeruptorum Dunn and Peucedanum decursivum (Miq.) Maxim. The core markers of Peucedanum praeruptorum Dunn were praeruptorin A and praeruptorin B in linear furanocoumarins, while Peucedanum decursivum (Miq.) Maxim. was rich in isodecursinol and hydroxylated derivatives, such as 7-hydroxycoumarin, in angular pyranocoumarins. Conclusion The UPLC-MS/MS method devised in this study has proved to be highly efficient and precise. Along with chemometric models, this method can shed light on the species-specific distribution profiles of coumarins in Peucedanum medicinal materials. This approach offers a multi-index collaborative control strategy as well as data-driven means for the varietal authentication, quality standardization, and regulatory supervision of traditional Chinese medicines.

Key words: Peucedanum praeruptorum Dunn, Peucedanum decursivum (Miq.) Maxim., Praeruptorin A, Praeruptorin B, Nodakenin, Coumarin, UPLC-MS/MS, Chemometrics

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