中国药物警戒 ›› 2025, Vol. 22 ›› Issue (4): 377-380.
DOI: 10.19803/j.1672-8629.20240848

• 液相色谱质谱联用法在药物临床评价中的应用研究专栏 • 上一篇    下一篇

高效液相色谱质谱联用法测定血浆中利奈唑胺浓度

王佳庆1, 汪祺2,△, 刘彤彤3, 张冬婕3, 陈启4, 梅升辉3, 宁霄5,*   

  1. 1首都医科大学附属北京天坛医院临床试验中心,北京 100050;
    2中国食品药品检定研究院中药民族药检定所,北京 100050;
    3首都医科大学附属北京天坛医院药学部,北京 100050;
    4浙江树人学院树兰国际医学院,浙江 杭州 310000;
    5中国食品药品检定研究院食品化妆品检定所,北京 100050
  • 收稿日期:2024-11-01 发布日期:2025-04-17
  • 通讯作者: *宁霄,女,硕士,主任药师,分析化学研究。
  • 作者简介:王佳庆,女,硕士,副主任药师,临床试验与药物分析研究。△为并列第一作者。
  • 基金资助:
    国家重点研发计划（2021YFC2401100）

Determination of Linezolid Concentrations in Plasma with HPLC-MS/MS Method

WANG Jiaqing1, WANG Qi2,△, LIU Tongtong3, ZHANG Dongjie3, CHEN Qi4, MEI Shenghui3, NING Xiao5,*   

  1. 1Clinical Trial Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;
    2Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institute of Food and Drug Control, Beijing 100050, China;
    3Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;
    4Shulan International Medical College of Zhejiang Shuren University, Hangzhou Zhejiang 310000, China;
    5Institute for Food and Cosmetics Testing, National Institutes for Food and Drug Control, Beijing 100050, China
  • Received:2024-11-01 Published:2025-04-17

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摘要： 目的 建立高效液相质谱联用法（HPLC-MS/MS）测定血浆中利奈唑胺浓度的检测方法,为血药浓度监测提供参考。方法 以利奈唑胺-D3为内标,加入含有内标的样本释放剂后用甲醇沉淀蛋白后取上清液进样检测。结果 利奈唑胺在0.42~28 μg·mL-1内线性良好;低浓度（25.2 μg·mL-1）的平均回收率为106.7%,中浓度（140 μg·mL-1）的平均回收率为104.9%,高浓度（210 μg·mL-1）的平均回收率为105.9%。日内和日间准确度2.38%~6.74%,精密度在1.37%~2.78%。结论 建立简便、快速利奈唑胺的分析方法,可用于临床快速的血药浓度监测。

关键词: 利奈唑胺, 液相色谱-质谱联用, 治疗药物监测, 血浆, 牛血清

Abstract: Objective To establish an HPLC-MS/MS method for determining the concentration of linezolid in plasma. Methods Linezolid-D3 was used as the internal standard. The sample release agent containing the internal standard was added. Protein was precipitated with methanol before the supernatant was injected for detection. Results Linezolid showed good linearity within the range of 0.42-28 μg·mL-1. The average recovery was 106.7% for the low concentration, 104.9% for the medium concentration, and 105.9% for the high concentration. The intra-day and inter-day precision was 2.38 %-6.74 % and 1.37 %-2.78 %, respectively, and the precision ranged from 1.37 % to 2.78 %. Conclusion This method is simple and fast, which can be used for rapid clinical monitoring of blood drug concentrations.

Key words: Linezolid, Liquid Chromatography-Mass Spectrometry, Therapeutic Drug Monitoring, Plasma, Bovine Serum

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