中国药物警戒 ›› 2011, Vol. 8 ›› Issue (12): 713-715.

• 基础及临床研究 • 上一篇    下一篇

毒性导向下的北豆根中蝙蝠葛碱的质量控制方法学研究

郑丽娜1, 2, 孙蓉1,*, 杨倩3   

  1. 1 山东省中医药研究院,山东 济南 250014;
    2 天津中医药大学,天津 300193;
    3 山东中医药大学,山东 济南250355
  • 收稿日期:2011-03-03 出版日期:2011-12-10 发布日期:2015-07-30
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理研究。E-mail:sunrong107@163.com
  • 作者简介:郑丽娜,女,在读硕士研究生,药物分析与中药毒代研究。
  • 基金资助:
    国家重点基础研究发展计划 (973)中医基础理论专项资助项目 (2009CB522802); 山东省科技平台建设项目(2008GG2NS02021)

Study on Quality Control Methodology of Menispermine from Rhizoma Menispermi under the Guidance of Toxicity

ZHENG Li-na1, 2, SUN Rong1, *, YANG Qian3   

  1. 1 Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2 Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    3 Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2011-03-03 Online:2011-12-10 Published:2015-07-30

摘要: 目的 以北豆根不同组分的小鼠急毒研究为导向,进行北豆根毒性物质基础蝙蝠葛碱的质量控制方法学研究。方法 用高效液相色谱法测定北豆根全组分、水提组分、醇提组分中蝙蝠葛碱的含量。色谱条件:色谱柱用十八烷基硅烷键合硅胶为填充剂(250mm×4.6mm,5μm),流动相:乙腈- 0.05%三乙胺水溶液(40:60),流速:1.0mL/min,检测波长:282nm,柱温:25℃。结果 蝙蝠葛碱在0.82~9.84 g范围内与其峰面积呈良好的线性关系,平均回收率为98.7%。对北豆根不同组分中的蝙蝠葛碱的含量测定,发现蝙蝠葛碱的含量大小为:全组分>水提组分>醇提组分。结论 高效液相色谱法测定北豆根中蝙蝠葛碱的含量,方法线性关系良好,精密度、稳定性、重现性均较好,回收率较高。北豆根不同组分中蝙蝠葛碱的含量大小与急性毒性间均有一定的关系,但不完全一致。初步提示中药成分复杂,蝙蝠葛碱不是北豆根中的唯一毒性物质基础,因此寻找其他毒性成分进行多成分指标控制北豆根质量,对于北豆根的毒性研究具有重要作用。

关键词: 北豆根, 蝙蝠葛碱, 急毒, 高效液相色谱法

Abstract: Objective Under the guidance of the acute toxicity of different components of Rhizoma Menispermi, the quality control methodology of Menispermine from Rhizoma Menispermi was studied. Methods HPLC was used to determine the content of Menispermine in all-components, water extract,alcohol extract of Rhizoma Menispermi. The C18 column(250mm×4.6mm,5μm)with the solvent system consisting of acetonitrile -0.05% triethylamine(40:60)was used.The detection wavelength was at 282nm by UV spectrometry and the column temperature was 30℃. The flow rate was 1.0mL/min. Results During 0.82~9.84 g, the linear regression of menispermine was favorable. The average recovery rates were 98.7%. In the determination of Menispermine in different components of Rhizoma Menispermi, it was found that the size order of the content of Menispermine was all-component > water extract > alcohol extract. Conclusion The method that Menispermine was determined by HPLC had good linearity, precision, stability and reproducibility, high recovery. There was a certain relationship between the content of Menispermine and acute toxicity in different components of Rhizoma Menispermi, but not completelyconsistent. It was proved that Chinese medicine was complex, and Menispermine was not the only toxic material basis of Rhizoma Menispermi. So Looking for other toxic ingredients to control the quality of Rhizoma Menispermi by more component index had an important role in the toxicity study of Rhizoma Menispermi.

Key words: Rhizoma Menispermi, Menispermine, acute toxicity, HPLC

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