中国药物警戒 ›› 2018, Vol. 15 ›› Issue (11): 641-643.

• 基础与临床研究 •    下一篇

糖尿病胃轻瘫大鼠模型的建立

黄举凯, 程淑莉, 杨晓晖*   

  1. 北京中医药大学东方医院内分泌科,北京 100078
  • 收稿日期:2019-01-09 修回日期:2019-01-09 出版日期:2018-11-20 发布日期:2019-01-09
  • 通讯作者: 杨晓晖,男,博士,主任医师,教授,内分泌系统疾病的中医药治疗。E-mail:yxh0616@126.com
  • 作者简介:黄举凯,男,在读博士,内分泌系统疾病的中医药治疗。
  • 基金资助:
    北京市科委“十病十药”研发专项课题（Z171100001717015）：十病十药研发-治疗糖尿病胃轻瘫中药复方佛香散的临床前研究; 北京中医药大学基本科研业务费项目（2018-JYBZZ-XS197）：佛香散对糖尿病胃轻瘫大鼠脑组织的病理组织形态及纹状体多巴胺D2受体影响观察

Establishment of Diabetic Gastroparesis Rat Model

HUANG Jukai, CHENG Shuli, YANG Xiaohui*   

  1. Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China
  • Received:2019-01-09 Revised:2019-01-09 Online:2018-11-20 Published:2019-01-09

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摘要： 目的 采用SD大鼠高脂高糖饮食联合腹腔注射链脲菌素（STZ）造模,观察糖尿病大鼠的胃排空功能,探索建立糖尿病胃轻瘫（DGP）大鼠模型的方法。方法 采用高脂高糖饲料（45%脂肪）喂养2周,禁食、自由饮水12 h后腹腔注射50 mg·kg-1 STZ的方法建立糖尿病大鼠模型。随机选择10只正常SD大鼠作为正常组,10只造模成功的SD大鼠作为模型组。两组大鼠均予普通饲料喂养8周后取鼠胃,检测胃排空及小肠推进率。结果 模型组大鼠形体消瘦,体重减轻,摄食增加。与正常组比较,模型组大鼠血糖水平均明显升高（P<0.05）,胃残留物的重量明显增加（P <0.05）,胃排空率显著下降。模型组大鼠小肠推进率与血糖呈负相关（相关系数r=-0.46,P=0.041）。结论 高脂高糖饮食联合STZ腹腔注射的方法建立糖尿病大鼠模型后普通饲料喂养8周可成功建立DGP大鼠模型,可成为DGP动物实验研究经济、简便、易行的造模方法。

关键词: 高脂高糖, 糖尿病大鼠, 胃轻瘫, 胃排空

Abstract: Objective To observe the gastric motility of diabetic rats and to explore a method of establishing a diabetic gastroparesis (DGP) rat model by feeding diabetic rats with high fat and high sugar diet combined with intraperitoneal injection of STZ. Methods To establish diabetic rat model, normal SD rats were fed with high fat and high sugar diet (45% fat content) for 2 weeks; then streptozocin (STZ) 50 mg·kg-1 was intraperitoneally injected after fasting and free drinking water for 12 hours. Ten normal SD rats were randomly selected for normal group, and 10 SD rats modeled successfully for model group. Stomachs of rats in the 2 groups were taken after 8 weeks of common feeding to measure gastric emptying and intestinal propulsion rate. Results Rats in the model group had thin physique, weight loss, and increased food intake. Compared with the normal group, the blood glucose level of the model group was significantly higher (P <0.05), the weight of the gastric residue was significantly increased (P<0.05), and the gastric emptying was significantly decreased. There was a negative correlation between the rate of intestinal propulsion in the model group (correlation coefficient r=-0.46, P =0.041). Conclusion Diabetic rat modeled by administrating high fat and high sugar diet feeding combined with STZ intraperitoneal injection and then administrating normal feeding for 8 weeks can establish a DGP rat model, which can be an economical, simple, and feasible modeling method for DGP animal experiments.

Key words: high fat and high sugar diet, diabetic rats, gastroparesis, gastric emptying

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