中国药物警戒 ›› 2016, Vol. 13 ›› Issue (10): 595-597.

• 质量与工艺研究 • 上一篇    下一篇

银黄片中黄芩苷含量测定方法探讨

郑艳青, 刘向国, 张静, 马斐, 宋秀荣   

  1. 山东省德州市食品药品检验检测中心,山东 德州 253000
  • 收稿日期:2016-11-23 修回日期:2016-11-23 出版日期:2016-10-20 发布日期:2016-11-23
  • 作者简介:郑艳青,女,本科,副主任中药师,中药及中成药的检验。

Discussion on Method of Content Determination of Baicalin in Yinhuang Tablets

ZHENG Yan-qing, LIU Xiang-guo ,ZHANG Jing, MA Fei ,SONG Xiu-rong   

  1. Dezhou Food and Drug Control/Testing Center , Shandong Dezhou 253015, China
  • Received:2016-11-23 Revised:2016-11-23 Online:2016-10-20 Published:2016-11-23

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摘要： 目的 通过对比试验来改进银黄片质量标准中黄芩苷提取物的含量测定方法 。方法 样品经处理后,采用HPLC法,色谱柱为C18（250 mm×4.6 mm×5 μm）;流动相为甲醇-水-磷酸（50：50：0.2）;流速为1.0 mL·min-1,柱温为30℃;检测波长为274 nm。结果 黄芩苷在14.26~85.54 μg·mL-1浓度范围内线性关系良好,平均加样回收率为98.47%（RSD=1.12%）。结论 修改后的分析方法可靠准确,操作简单,分离效果好,灵敏度高,更加适用于银黄片的质量控制方法。

关键词: 银黄片, 黄芩苷, 高效液相色谱法

Abstract: Objective To improve the method by comparative experiment discussion on method of content determination of Baicalin in Yinhuang Tablets. Methods Samples were extracted by 50%-methanal, then analyzed on C18 chromatography column (250 mm× 4.6 mm× 5 μm)with mobile phase consisted methanol-purified water-phosphoric acid (50:50:0.2) at the flow rate of 1.0 mL·min-1. The column temperature was maintained at 30℃ and the detection wavelength was set at 274 nm. Results Good linearig of baicalin was achieved in the concentration range of 14.26 ~ 85.54 μg·min-1 (r=0.999 6) and the average recovey was 98.47% (RSD=1.12%). Conclusion The improved method is reliable and accurate, simple and sensitive with good separation efficiency. It is more suitable for the quality control of Yinhuang tablets.

Key words: Yinhuang tablet, baicalin, HPLC

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