中国药物警戒 ›› 2013, Vol. 10 ›› Issue (10): 594-599.

• 附子急性毒性研究 • 上一篇    下一篇

附子不同组分对疼痛小鼠的急性毒性实验比较研究

孙蓉1, 栾永福2   

  1. 1.山东省中医药研究院,山东济南 250014；
    2.天津中医药大学,天津 300073
  • 收稿日期:2013-09-17 修回日期:2016-03-09 出版日期:2013-10-08 发布日期:2016-03-09
  • 作者简介:孙蓉,女,研究员·博导,博士,中药药理与毒理。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目课题2（2009CB522802）。

Comparative Study on Acute Toxicity of Different Components of on Algesic Mice

SUN Rong1 ,LUAN Yong-fu2   

  1. 1.Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2.Tianjin University of Traditional Chinese Medicine, Tianjin 300073, China
  • Received:2013-09-17 Revised:2016-03-09 Online:2013-10-08 Published:2016-03-09

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摘要： 目的 比较附子不同组分对疼痛小鼠急性毒性的影响,进行附子药材的急性毒性观察与评价,为临床安全合理用药提供依据。方法 用0.6%冰醋酸制作疼痛小鼠模型,分别制备附子全组分、水提组分和醇提组分,采用经典的急性毒性实验方法分别测定最大耐受量（MTD）和半数致死量（LD50）。结果 附子醇提组分的对疼痛小鼠的LD50 值为13.6 g·kg-1·d-1,相当于临床70.0kg 的成人每公斤体重日用量的63.5 倍；附子全组分、水提组分对疼痛小鼠的MTD为8.0 g·kg-1·d-1 和14.4 g·kg-1·d-1,相当于临床70.0kg 的成人每公斤体重日用量的37.3 倍和67.2 倍。通过观察发现,附子灌胃小鼠的急性毒性症状主要有：怠动、恶心、四肢麻痹、抽搐、惊厥和俯卧不动,且多发生于6.0h之内。结论 附子不同组分对疼痛小鼠具有一定的急性毒性,其毒性强度大小为：醇提组分>全组分>水提组分。

关键词: 附子, 小鼠, 急性毒性

Abstract: Objective To compare the acute toxicity effect of different components of Aconite on algesic mice and observe/evaluate the acute toxicity of Aconite herbs. Thus, we try to provide a foundation for rational drug use in clinic. Methods We made algesic mice model with 0.6% acetic acid, prepared the all -components, water extracted components and alcohol extracted components, then ig. all -components, water extracted components and alcohol extracted components of Aconite to mice and the MTD, LD50 were tested respectively by the classic Methods of acute toxicity. Results We found that the LD50 of alcohol extracted components was 13.6g·kg-1·d-1, which was equal to 63.5 times of the clinical daily dosage in 70kg humans. The MTD of all-components and water extracted components was respectively 8.0g·kg-1·d-1 and 14.4g·kg-1·d-1, which was respectively equal to 37.3 times and 67.2 times of the clinical daily dosage in 70 kg humans. By observing, we found that hypokinesia, nausea, quadriplegia, hyperspasmia, consequence and prostrate motionlessness were the main acute toxical symptoms caused by Aconite after gavage adminis-tration, and these symptoms mostly occurred in six hours. Conclusion Different components of Aconite could cause certain acute toxicity effect on algesic mice, and the alcohol extracted components were with the greatest degree of toxicity followed by the all-components, and the least was the water extracted components.

Key words: mouse, acute toxicity

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