甲基莲心碱对LPS诱导人脐血管内皮细胞损伤中NF-κB信号通路及相关因子表达的影响

中国药物警戒 ›› 2017, Vol. 14 ›› Issue (3): 129-132.

• 基础与临床研究 • 下一篇

甲基莲心碱对LPS诱导人脐血管内皮细胞损伤中NF-κB信号通路及相关因子表达的影响

戴谆, 宋金春^*

武汉大学人民医院药学部,湖北武汉 430060

收稿日期:2017-04-20 修回日期:2017-04-20 出版日期:2017-03-20 发布日期:2017-04-20
通讯作者: 宋金春,男,教授,临床药学。E-mail: songjc1234@126.com
作者简介:戴谆,女,在读硕士,临床药学。

Effect of Neferine on Expression of NF-κB Signaling Pathway and Correlation Factors in LPS-induced Human Umbilical Vein Endothelial Injury

DAI Zhun, SONG Jin-chun^*

Dept of Pharmacy, Renmin Hospital of Wuhan University, Hubei Wuhan 430060, China

Received:2017-04-20 Revised:2017-04-20 Online:2017-03-20 Published:2017-04-20

摘要/Abstract

摘要： 目的观察甲基莲心碱对LPS诱导的与平滑肌细胞共培养的人脐血管内皮细胞中NF-κB信号通路及相关黏附因子的表达的影响,探讨甲基莲心碱抗动脉粥样硬化的分子机制。方法 Transwell板建立人脐血管内皮细胞及平滑肌细胞共培养体系后,通过脂多糖（LPS）建立损伤模型,分别用含梯度浓度（0、0.1、0.3、1、3 µmol·L^-1）甲基莲心碱的培养液培养24 h。采用免疫印迹法（Western blot）检测人脐血管内皮细胞（HUVECs）内NF-κB信号通路相关蛋白表达的变化;采用实时荧光定量PCR方法检测黏附因子TNF-ɑ和细胞间黏附因子-1（ICAM-1）mRNA的相对表达。结果在甲基莲心碱干预LPS诱导损伤的VEC-SMC共培养体系后,降低了NF-κB p65的表达,提高IκB-ɑ的表达;下调TNF-ɑ和ICAM-1相关炎性因子的表达,且成一定的浓度依赖性。结论甲基莲心碱通过抑制血管内皮细胞NF-κB信号通路,下调相关炎性因子的表达,减少炎症反应,从而抗动脉粥样硬化。

关键词: 甲基莲心碱, 脂多糖, 人脐血管内皮细胞, NF-κ, B信号通路, 炎性因子

Abstract: Objective To observe the anti-atherosclerosis effect of neferine on activiation of NF-κB and correction factors by establishing of VEC-SMC co-culture system induced by LPS. Methods An indirect co-cultivating system between VEC and SMC was constructed by planting VEC in Transwell cell’s upper chamber and SMC in lower chamber. The injuring condition of VEC was built after stimulating VEC using LPS, which was treated with neferine in different concentrations (0, 0.1, 0.3, 1, 3 μmol·L^-1) for 24 h. The protein levels of IκB-ɑ, NF-κB p65 were detected by Western blotting. The mRNA expression of TNF-ɑ and ICAM-1 were determined by RT-PCR. Results After treated with Nef, the expression of NF-κB p65, TNF-ɑ mRNA and ICAM-1 mRNA in HUVECs injured by LPS were decreased, while the expression of IκB-ɑ was increased in a concentration-dependent manner. Conclusion Neferine may inhibit NF-κB signaling pathway and decrease the expression of correction factors, in order to reduce the inflammatory reaction in HUVECs and alleviate the atherosclerosis.

Key words: neferine, LPS, HUVECs, NF-kappa B signaling pathway, inflammatory factor

中图分类号:

戴谆, 宋金春. 甲基莲心碱对LPS诱导人脐血管内皮细胞损伤中NF-κB信号通路及相关因子表达的影响[J]. 中国药物警戒, 2017, 14(3): 129-132.

DAI Zhun, SONG Jin-chun. Effect of Neferine on Expression of NF-κB Signaling Pathway and Correlation Factors in LPS-induced Human Umbilical Vein Endothelial Injury[J]. Chinese Journal of Pharmacovigilance, 2017, 14(3): 129-132.

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甲基莲心碱对LPS诱导人脐血管内皮细胞损伤中NF-κB信号通路及相关因子表达的影响

Effect of Neferine on Expression of NF-κB Signaling Pathway and Correlation Factors in LPS-induced Human Umbilical Vein Endothelial Injury

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