谷红注射液改善脑缺血再灌注大鼠线粒体功能的组方合理性探讨

中国药物警戒 ›› 2016, Vol. 13 ›› Issue (12): 708-711.

谷红注射液改善脑缺血再灌注大鼠线粒体功能的组方合理性探讨

冯璐¹, 左萍萍^1,*,王明洋¹,杨楠¹,范思远²,崔丽英²

1 中国医学科学院基础医学研究所药理室,北京 100005;
2 北京协和医院神经内科,北京 100005

收稿日期:2017-01-13 修回日期:2017-01-13 出版日期:2016-12-20 发布日期:2017-01-13
通讯作者: 左萍萍,女,博士生导师,神经药理学。E-mail：zuopp@vip.126.com
作者简介:冯璐,女,硕士研究生,中枢神经药理。
基金资助:
国家自然科学基金项目(81100801)

Exploration on Reasonable Prescription of Guhong Injection in Improving Mitochondrial Function of Cerebral Ischemia-reperfusion Rats

FENG Lu¹, ZUO Ping-ping^{1, *}, WANG Ming-yang¹, YANG Nan¹, FAN Si-yuan², CUI Li-ying²

1 Department of Pharmacology, Institute of Basic Medical Sciences, Beijing 100005, China;
2 Department of Neurology, Peking Union Medical College Hospital /Chinese Academy Medical Sciences, Beijing 100005, China

Received:2017-01-13 Revised:2017-01-13 Online:2016-12-20 Published:2017-01-13

摘要/Abstract

摘要： 目的探究谷红注射液及其主要成分乙酰谷酰胺和红花对脑缺血再灌注损伤大鼠线粒体自噬功能相关蛋白表达的差异。方法采用线栓法制备SD大鼠大脑中动脉梗阻（MCAO）复灌模型,随机分为假手术组、模型组、乙酰谷酰胺组、红花组和谷红组（n=8）。术后即刻给药,连续给药14 d后通过神经功能缺损症状评分及贴纸去除试验评价药效。采用Western blot方法检测线粒体自噬相关蛋白Beclin1、Parkin、KIFC2及UCP3的表达变化。结果与对照组相比,模型组大鼠神经功能缺损症状明显（P<0.05）,贴纸去除时间明显延长（P<0.05）;谷红治疗组于给药后14 d显著减轻缺血大鼠神经功能缺损症状（P<0.01）,并缩短贴纸去除时间（P<0.05）,而乙酰谷酰胺组和红花组未见明显改变;谷红治疗还能有效逆转缺血大鼠大脑皮层线粒体与自噬功能相关的前述蛋白表达的变化（P<0.01）,而乙酰谷酰胺组和红花组无明显改变。结论谷红注射液有显著改善脑卒中大鼠线粒体功能的效果,其疗效明显强于单独注射乙酰谷酰胺和红花。

关键词: 谷红注射液, 乙酰谷酰胺, 红花, 脑缺血再灌注, 线粒体自噬

Abstract: Objective To explore the difference of the expression of autophagy related proteins between Guhong injection and its main components acetylglutamine and safflower in rats with cerebral ischemia reperfusion injury. Methods The middle cerebral artery occlusion (MCAO) model of SD rats was made by thread embolization. The rats were randomly divided into sham operation group, model group, acetylglutamine group, safflower group and Guhong group (n = 8). Behavioral tests (modified Neurological Severity Score and adhesive-removal test) were performed at 14 d after MCAO. The expressions of Beclin1, Parkin, KIFC2 and UCP3 were detected by Western blot. Results Significant changes in Neurological Severity Score, somatosensory and motor behavior were found in model animals, but there were no obvious change in the groups of acetylglutamate and safflower. The treatment of Guhong could effectively reverse cortical mitochondria and autophagy function of the above-mentioned protein expression changes in ischemic rats (P <0.01), while the acetylglutamine group and safflower group had no significant change. Conclusion The effect of Guhong injection on function of mitochondria of cerebral ischemia reperfusion injury in rats was significantly stronger than acetylglutamine injection and safflower injection.

Key words: Guhong injection, acetylglutamine, safflower, brain ischemia, mitophagy

中图分类号:

R965

冯璐, 左萍萍,王明洋,杨楠,范思远,崔丽英. 谷红注射液改善脑缺血再灌注大鼠线粒体功能的组方合理性探讨[J]. 中国药物警戒, 2016, 13(12): 708-711.

FENG Lu, ZUO Ping-ping, WANG Ming-yang, YANG Nan, FAN Si-yuan, CUI Li-ying. Exploration on Reasonable Prescription of Guhong Injection in Improving Mitochondrial Function of Cerebral Ischemia-reperfusion Rats[J]. Chinese Journal of Pharmacovigilance, 2016, 13(12): 708-711.

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