中国药物警戒 ›› 2015, Vol. 12 ›› Issue (7): 389-393.

• 基础及临床研究 • 上一篇    下一篇

浊度法测量血小板聚集的变异分析及其在药效评价中的应用

毛鑫1,2, 韩淑娴2, 赵海娟1,2, 张兰珍1, 游云2, 廖福龙2   

  1. 1. 北京中医药大学中药学院,北京 100102;
    2. 中国中医科学院中药研究所,北京 100700
  • 收稿日期:2015-09-01 修回日期:2015-09-01 出版日期:2015-07-08 发布日期:2015-09-02
  • 通讯作者: 游云,女,博士,研究员·硕导,中药药理学。E-mail：youyunrice@126.com
  • 作者简介:毛鑫,男,在读硕士,中药学。
  • 基金资助:
    国家自然科学基金资助项目（81274006,81473548）

Variability Analysis of Turbidity Measurement of Platelet Aggregation and Its Application in Efficacy Evaluation

MAO Xin1,2, HAN Shu-xian2, ZHAO Hai-juan1,2, ZHANG Lan-zhen1, YOU Yun2, LIAO Fu-long2   

  1. 1. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China;
    2. Institute of Traditional Chinese Medicine, China Academy of Traditional Chinese Medicine, Beijing 100700, China
  • Received:2015-09-01 Revised:2015-09-01 Online:2015-07-08 Published:2015-09-02

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摘要： 目的 考察浊度法测量血小板聚集指标的稳定性及其在药效评价中的应用。方法 采用北京泰利康信血小板聚集仪（LBY-NJ4）检测不同诱导剂诱导血小板聚集参数的变异系数（CV）,评价阿司匹林和三七总皂苷抑制血小板聚集的效应及其稳定性。结果 在二磷酸腺苷（ADP）和胶原诱导剂作用下,血小板最大聚集率、达峰速率和聚集曲线下面积的变异系数均分别小于4%、4%和7%。凝血酶作用下的聚集达峰速率变异系数偏大为24.45%。排除纤维蛋白的影响,选择洗涤血小板观察凝血酶诱导聚集作用,达峰速率的变异幅度降低至4.42%。三七总皂苷以剂量依赖方式抑制血小板聚集,3项聚集参数的CV值在1.58%~12.34%之间,与阿司匹林相比表现出较好的稳定性。结论 利用浊度法测量血小板聚集,3项聚集参数稳定性较好;并利用多种参数评价药物抑制血小板聚集的效应及其稳定性,可以更好地发现活血化瘀中药的作用特点。

关键词: 浊度法, 血小板聚集, 变异系数

Abstract: Objective To evaluate turbidity measurement which is used to study the variability and variation amplitude of platelet aggregation and its application. Methods LBY-NJ4 aggregometer was use to test coefficients of variation (CV) of different inducers which induce platelet aggregation, evaluate the effect and variability of aspirin and panax notoginseng (PNs) inhibition of platelet aggregation. Results With adenosine diphosphate (ADP) and collagen, the CV of maximum aggregation rate, aggregation rate and area under the curve were less than 4%, 4% and 7% respectively, while with thrombin the CV of aggregation rate was larger(24.45%). Washed platelet was engaged to the aggregation of thrombin to remove the effect of fibrin, the CV of aggregation rate was reduced to 4.42%. PNs inhibited thrombin-induced platelet aggregation with a dose-depend manner, the CVs of three parameters were ranged from 1.58% to 12.34%, more stable than that of aspirin. Conclusion Turbidity measurement can be used to test platelet aggregation, three parameters are stable and useful for the evaluation of traditional Chinese medicine for huoxuehuayu.

Key words: turbidity measurement, platelet aggregation, coefficient of variation

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