中国药物警戒 ›› 2013, Vol. 10 ›› Issue (1): 8-11.

• 参莪汤抗肿瘤作用研究 • 上一篇    下一篇

参莪汤有效组分体内抗肿瘤作用量-效关系研究

谢元璋12, 孙蓉2*,张亚囡3,李晓宇3,季宇彬1*   

  1. 1.哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076；
    2.山东省中医药研究院,山东济南250014；
    3.山东中医药大学,山东济南250355
  • 收稿日期:2012-11-07 修回日期:2016-03-12 出版日期:2013-01-08 发布日期:2016-03-09
  • 通讯作者: 季宇彬,男,博士,教授,博士生导师,抗肿瘤药物。 E-mail：jyb@hrbcu.edu.cn
  • 作者简介:孙蓉,女,博士,研究员,博士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 基金资助:
    国家重大新药创制专项课题：中药复方药理学研究与药效评价关键技术(2009ZX09502-015)

Study on the "Dose-efficacy" Relationship of Anti-tumor Induced by Effective Component from Shenetang

XIE Yuan-zhang1,2 ,SUN Rong2* ,ZHANG Ya-nan3, LI Xiao-yu3, JI Yu-bin1   

  1. 1..Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Heilongjiang Harbin 150076, China;
    2..Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    3..Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2012-11-07 Revised:2016-03-12 Online:2013-01-08 Published:2016-03-09
  • Supported by:
    谢元璋,男,在读硕士,药理学。

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摘要： 目的 探讨参莪汤有效组分对肝癌H22肿瘤小鼠体内抗肿瘤的“量- 效”关系,为新药开发提供客观实验依据。方法 采用H22腹水瘤细胞接种的方法,建立H22肿瘤小鼠动物模型,给予不同剂量的参莪汤有效组分,连续灌胃给药9 天,末次给药后24h,取瘤块、胸腺、脾脏,计算抑瘤率和脏体比值。观察药物对H22 荷瘤小鼠的抑瘤作用,碳粒廓清检测对小鼠单核巨噬细胞吞噬功能的影响。结果 通过对各组小鼠抗肿瘤作用分析,参莪汤有效组分在8.31～70.72 g·kg-1剂量范围内能抑制肝癌H22小鼠肿瘤生长,增加小鼠脾指数和胸腺指数,增强小鼠单核巨噬细胞的吞噬功能。结论 参莪汤有效组分在8.31～34.65 g·kg-1剂量范围内具有抗肿瘤作用,呈现一定“量- 效”关系,其抗肿瘤作用的最佳剂量为34.65 g·kg-1,最小有效量为8.31 g·kg-1,在一定剂量范围内,随剂量增加,抑制作用增强,呈现一定的剂量依赖关系,达到最高效应时,抗肿瘤作用不再随剂量增加而增强。参莪汤有效组分通过免疫调节提高小鼠免疫功能达到抑制肿瘤作用。

关键词: 参莪汤, 量效关系, 有效组分

Abstract: Objective To explore anti -tumor effect of effective components from shenetang , so as to provide experimental basis for the development of new drugs. Methods H22 tumor model in mice was built. Mice implanted withH22 were treated with effective components from shenetang for 7d, then the tumor tissues, thymus glands and spleens were weighed. The tumor inhibitory rates, immune organ index and carbon granule clearance index were calculated to investigate the anti -tumor and immune regulatory effect of effective components from shenetang in mice implanted with tumor cells. Results Effective components from shenetang showed more potent anti-tumor effect within the dose range in 8.31～70.72g·kg-1, it could raise the decreased immune organ index and carbon granule clearance index. Conclusion Effective components from shenetang in 8.31～34.65g·kg-1 dose range shows a certain "dose-efficacy" relationship of anti-tumor activity. The optimal dose of anti-tumor effect is 34.65g·kg-1 and the minimum effective dose is 8.31g·kg-1. With the dose increasing, ina certain dose range, the inhibition enhanced, it shows dose dependency. When it achieves maximum effect, the anti-tumor effect no longer increases. The effective component can improve immune function to inhibit tumor.

Key words: Shenetang, "dose-efficacy" relationship, effective component

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