中国药物警戒 ›› 2013, Vol. 10 ›› Issue (1): 12-15.

• 参莪汤抗肿瘤作用研究 • 上一篇    下一篇

参莪汤不同组分抗肿瘤活性筛选研究

吕莉莉1,谢元璋12, 张亚囡3,朱兰兰1,季宇彬2,孙蓉1   

  1. 1.山东省中医药研究院,山东济南250014；
    2.哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076；
    3.山东中医药大学,山东济南250355
  • 收稿日期:2016-03-12 修回日期:2016-03-12 出版日期:2013-01-08 发布日期:2016-03-09
  • 通讯作者: 孙蓉,女,博士,研究员,博士生导师,中药药理与毒理。 E-mail：sunrong107@163.com
  • 作者简介:吕莉莉,女,副研究员,中药药理与毒理。
  • 基金资助:
    国家重大新药创制专项课题：中药复方药理学研究与药效评价关键技术（2009ZX09502-015）

Study on the Anti-tumor Activity of Different Components from Shenetang

LV Li-li1 ,XIE Yuan-zhang1,2 ,ZHANG Ya-nan3 ,ZHU Lan-lan1 ,JI Yu-bin2 ,SUN Rong1   

  1. 1.Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2.Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Heilongjiang Harbin 150076, China;
    3.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2016-03-12 Revised:2016-03-12 Online:2013-01-08 Published:2016-03-09

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摘要： 目的 为研发低毒高效的抗肿瘤制剂,以抗肿瘤活性为先导,化学物质基础含量为指标,比较5 种提取物的药效强度,确定复方参莪汤有效组分的提取工艺,为进一步优化处方药学工艺奠定基础。方法 结合复方中各药味化学成分和药理活性,确定5 中不同提取方法,考察不同提取物中物质基础含量；建立肝癌H22肿瘤小鼠动物模型,给予相同剂量不同工艺的参莪汤有效组分,连续灌胃给药9天,末次给药后1天,取瘤块、胸腺、脾脏,计算抑瘤率和脏体比值,考察药物对肝癌H22肿瘤小鼠的抑瘤作用。结果 通过对不同提取物小鼠抗肿瘤作用分析,参莪汤各工艺组均能抑制小鼠肝癌H22肿瘤生长,增加小鼠脾指数和胸腺指数,其中工艺4组的抗肿瘤作用最强。结论 参莪汤有效组分抗肿瘤作用的最佳工艺为工艺4 组,其抗肿瘤活性最佳,具有良好的抗肿瘤应用前景。

关键词: 参莪汤, 不同组分, 抗肿瘤活性

Abstract: Objective To explore anti-tumor effect of five different extraction process from shenetang in vivo, so as to provide foundation to optimize the extraction process of the prescription. Methods The chemical composition and pharmacological activity of the various ingredients in the compound were summed up to determinate the extraction methods, the contents of material basis in different of extracts were detected.H22 tumor model in mice were built. Mice implanted withH22 were treated with five different extraction processes from shenetang for 7d, and then the tumor tissues, thymus glands and spleens were weighed. The tumor inhibitory rates, immune organ index were calculated to investigate the anti -tumor and immune regulatory effect of different extraction components from shenetang in mice implanted with tumor cells. Results The five different extraction components showed potent antitumor effect, it could raise the decreased immune organ index, in which group four shows better anti-tumor activity.Conclusion The effective components from shenetang have anti-tumor effect, group four with more anti-tumoractivity, which has good anti-tumor application prospects.

Key words: Shenetang, different extraction components, anti-tumor activity

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