关键词: 血塞通注射液, 剪应力, 血小板, 黏附, 微流装置

Abstract: Objective To observe the real-time influence of Xuesaitong injection on the collagenI induced platelet adhesion and aggregation. Methods Bioflux1000 microfluidic device was employed in this study for the first time. The observation channels were coated with collagen I. SD rats underwent anesthesia and blood withdrawn from aortic vessels with 3.8% sodium citrate 1:9 anticoagulation. Whole blood with Calcein AM(4μM) fluorescent labeling for 1 hr. Different concentration of Xuesaitong Injection were added to whole blood incubated for 10 minutes in room tempreture. Whole blood was perfused from the wells at different shear stress for 5 minutes. Fluorescent micrographs were captured with the blood under flow using an inverted microscope and CCD Camera. Inhibition rates by medicine were calculate by percent of control. Results Exposure to collagenI mediated stable platelet adhesion and aggregation within two to three minutes under 10dyn/cm2 flow. The IC50 of Xuesaitong injection on inhibiting platelet adhesion and aggregation is 0.25mg/ml under 10dyn/cm2 flow. By curve estimate we found the logarithm relation between shear stress and efficacy(R2=0.97). Conclusion Xuesaitong injection could inhibit collagen-induced platelet adhesion and aggregation under flow; Shear stress showed impact on the efficacy of medicine; Bioflux1000 could be used to evaluate the efficacy of Chinese medicine.

Key words: Xuesaitong injection, shear stress, platelet, adhesion, microfluidic devices