中国药物警戒 ›› 2010, Vol. 7 ›› Issue (10): 585-589.

• 基础及临床研究 • 上一篇    下一篇

黄连解毒汤体内外抗肿瘤作用的实验研究

孙健12,于友华2,张郑瑶1, 张扬1, 周秋丽1   

  1. 1.吉林大学药学院,吉林长春130021；
    2.中国中医科学院医学实验中心,北京100700
  • 收稿日期:2010-08-04 修回日期:2016-03-12 出版日期:2010-10-08 发布日期:2016-03-09
  • 通讯作者: 周秋丽,女,教授,中药复方药效物质基础研究。E-mail: qiulizhoujl@yahoo.com.cn
  • 作者简介:孙健,男,博士,药物分析和肿瘤药理学研究。

Antitumor Activities of Huanglianjiedu Decoction in Vivo and in Vitro

SUN Jian1,2, YU You-hua2 ,ZHANG Zheng-yao1 ,ZHANG Yang1, ZHOU Qiu-li1   

  1. 1.School of Pharmacy, Jilin University, Jilin Changchun 130021, China;
    2.Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2010-08-04 Revised:2016-03-12 Online:2010-10-08 Published:2016-03-09

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摘要： 目的 观察黄连解毒汤的体内、体外抗肿瘤作用。方法 采用小鼠肉瘤S180 和小鼠前胃癌MFC 等移植性肿瘤模型进行体内实验,通过检测抑瘤率、胸腺指数、脾脏指数等指标观察黄连解毒汤的体内抑瘤作用及对免疫器官的影响。并用MTT 方法检测黄连解毒汤对小鼠S180、小鼠MFC、人胃癌SGC-7901、人肝癌SMMC-7721 等4 种瘤株的抑制作用。结果 黄连解毒汤大、中剂量组中对S180 荷瘤小鼠有抑制作用,大剂量组对MFC 荷瘤小鼠有抑制作用,抑瘤率分别为48.38%、33.77%、30.74%；对S180、MFC、SGC-7901 和SMMC-7721 细胞均有一定的细胞毒作用,IC50分别为99.73 mg·L-1、84.47 mg·L-1、153.32 mg·L-1和102.87 mg·L-1。结论 黄连解毒汤体内对S180、MFC 小鼠移植瘤有一定的抗肿瘤活性,体外对4 种肿瘤细胞也具有抑制作用。

关键词: 黄连解毒汤, 抗肿瘤作用, 体内, 体外

Abstract: Objective To study the antitumor activities of Huanglianjiedu Decoction(HLJDT) in vivo and in vitro. Methods Antitumor activities were tested in mice with experimental tumor S180 and MFC in vivo, thymus index, spleen index, tumor inhibitory rate were evaluated. Cell proliferation was assayed by using methyl thiazolyl tetrazolium (MTT) method in vitro. S180, MFC, SGC -7901 and SMMC -7721 cancer cells were incubated in culture media containing HLJDT. Results HLJDT showeda Antitumor activities on S180 and MFC in vivo. All of the HLJDT in different dosage groups could highly inhibit the proliferation of 4 cancer cells. Conclusion The HLJDT could inhibit the tumor S180, MFC in mice, the drug has obvious anticancer effects against S180, MFC, SGC -7901 and SMMC7721.

Key words: Huanglianjiedu Decoction(HLJDT), antitumor activity, in vivo, in vitro

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