中国药物警戒 ›› 2011, Vol. 8 ›› Issue (6): 330-332.

• 基础研究 • 上一篇    下一篇

熟地黄口服给药大鼠慢性毒性实验研究

马志会, 张静, 朱桃桃, 周昆   

  1. 天津中医药大学中医药研究院,天津市中药药理重点实验室,天津 300193
  • 收稿日期:2015-08-27 修回日期:2015-08-27 出版日期:2011-06-10 发布日期:2015-08-27
  • 作者简介:马志会,女,硕士研究生,中药毒理研究。

Experimental Study on Chronic Toxicity of Radix Rehmanniae in Rats by Oral Administration

MA Zhi-hui, ZHANG Jing, ZHU Tao-tao, ZHOU Kun   

  1. Institute of Traditional Chinese Medicine, Tianjin key laboratory of Chinese Medicine, Tianjin key laboratory of Chinese Medicine pharmacology, Tianjin University of TCM, Tianjin 300193, China
  • Received:2015-08-27 Revised:2015-08-27 Online:2011-06-10 Published:2015-08-27

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摘要： 目的 观察熟地黄的亚慢性毒性,评价熟地黄用于保健食品的长期食用的安全性。方法 大鼠连续灌服熟地黄60天,60天后解剖取肝、心、脾、肾、脑、胸腺、肾上腺、生殖器,计算脏器系数;腹主动脉取血进行血常规检查和血生化检测,并检测血清高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）。结果 熟地黄高（4.05g/kg）、中（2.70g/kg）、低（1.35g/kg）3个剂量组与对照组相比,体重组间的差异无显著性（P >0.05）;3个剂量组的大鼠肝脏系数有增高的趋势,其中高剂量组与对照组相比,差异有显著性（P<0.01）;3个剂量组的白细胞计数（WBC）均低于对照组, 且低剂量和中剂量有显著性差异,低剂量组（P<0.001）,中剂量组（P<0.05）。血小板计数（PLT）均高于对照组,但只有低剂量组有显著性（P<0.05）。3个剂量组的生化指标中总胆固醇（TC）、HDL-C、LDL-C与对照组相比,均明显增高且具有显著性。天门冬氨酸氨基转移酶（AST）的活性均低于对照组,其中高剂量组具有显著性（P<0.01）。结论 熟地黄长期服用对大鼠体内胆固醇代谢具有一定的影响,能升高血中胆固醇的量,其作用机制可能与参与胆固醇合成和分解的酶有关。

关键词: 熟地黄, 大鼠, 高胆固醇

Abstract: Objective To observe the sub-chronic toxicity of Radix Rehmanniae, and evaluate the safety of Radix Rehmanniae as health food for long-term use. Methods Rats were continuously fed with Radix Rehmanniae 60 days, after 60 days, dissecting and having the liver, heart, spleen, kidney, brain, thymus, adrenal, genital, and calculating coefficient of organs; blooding from abdominal aortic for hematologic examination and blood biochemical detection, at the same, detecting HDL-C and LDL-C in serum. Results Radix Rehmanniae high(4.05g/kg), middle(2.70g/kg), low(1.35g/kg)3 dose groups compared with control group, weight between groups had no significant difference(P>0.05 ); liver coefficients of three dose groups had increasing trend, the high-dose group compared with the control group, the difference was significant(P<0.01); 3 dose groups of WBC were lower than the control group, and the low-dose and middle-dose had significant difference , the low dose group(P<0.001), middle dose group(P<0.05). PLT was higher, but only low-dose group had significant difference(P<0.05). Three doses of biochemical indexes in TC, HDL-C, LDL-C, compared with control group, were significantly higher and significant. AST was lower than the control group, and the high-dose group was significant(P<0.01). Conclusion Long-term using Radix Rehmanniae has some influence on cholesterol metabolism in rats, and can increase the amount of cholesterol in the blood, its mechanism may be involved in synthetical and decomposed enzymes of cholesterol.

Key words: Radix Rehmanniae, rat, high cholesterol

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