中国药物警戒 ›› 2011, Vol. 8 ›› Issue (1): 20-22.

• 基础及临床研究 • 上一篇    下一篇

千金子及不同含油量千金子霜急性毒性比较

孙付军, 宋卫国, 李英霞   

  1. 山东省中医药研究院,山东 济南 250014
  • 收稿日期:2010-01-29 出版日期:2011-01-10 发布日期:2015-08-24
  • 通讯作者: 李英霞,女,副研究员,中药资源开发、中药质量控制及中药炮制研究。E-mail:Li-yx1964@163.com
  • 作者简介:孙付军,男,助理研究员,中药药理研究。
  • 基金资助:
    国家“十一五”支撑计划基金资助项目（2006BAI09B06–05）

Study on Acute Toxicity of Semen Euphorbiae Prior to and After Making Frostlike Powder

SUN Fu-jun, SONG Wei-guo, LI Ying-xia   

  1. Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China
  • Received:2010-01-29 Online:2011-01-10 Published:2015-08-24

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摘要： 目的 通过考察千金子霜脂肪油含量与其急性毒性的关系,探讨千金子霜合理的含油量限度标准。方法 以传统法制得千金子霜含油量为参考,采用经典急性毒性实验方法测定千金子、千金子脂肪油、脱脂千金子粉及不同含油量（22.3％、25.0％、28.4％）的千金子霜小鼠口服半数致死量,观察小鼠进行急性毒性症状及体重变化。在无法测得LD50情况下,又对不同含油量的千金子霜小鼠出现俯卧、食少、被毛潮湿等轻度中毒反应的时间和程度进行了比较。结果 千金子的毒性部位在于脂肪油,脂肪油累积剂量达96g/kg/d,千金子和千金子霜累积剂量达39.96g/kg/d动物均无死亡,无法测出LD50。小鼠口服含油量分别为22.3％、25.0％和28.4％的千金子霜出现俯卧、少动等轻度中毒反应的时间和程度略有差别,随含油量的增加出现轻度不适反应的时间略早,程度略重。结论 初步实验结果表明,千金子中的毒性部位在于脂肪油,腹泻、弓背、被毛潮湿是主要的急性中毒症状。去油制霜后毒性明显降低。根据急性毒性实验结果,结合制霜生产实际和传统法制备霜品的含油量范围,建议千金子霜含油量限度标准从18.0%~20.0%可适当放宽为18.0％~22.0％。

关键词: 千金子, 千金子霜, 急性毒性, 含油量范围

Abstract: ObjectiveTo study the relationship between acute toxicity of Semen Euphorbiae and the content of fatty oil, and the reasonable fatty oil standard of Semen Euphorbiae Pulveratun. MethodsIg Semen Euphorbiae, fatty oil in Semen Euphorbiae, Semen Euphorbiae Pulveratun having different fatty oil(0%, 22.3％, 25.0％and 28.4%) to mice and test the weight and LD50 of oral dose by the conven-tional method for measuring acute toxicity. We compared the time and degree appearing the slight ill symptom such as lying, less food, wetting fur in mice. ResultsAll of the mice tested survive and the LD50 of oral dose to mice is not possible to determine. The maximum dosage of fatty oil in mice is 96g/kg/d and is 39.96g/kg/d either Semen Euphorbiae or Semen Euphorbiae Pulveratun for mice. The time for the occurrence of slight symptom and the degree in mice given Semen Euphorbiae Pulveratun having different fatty oil(22.3%, 25.0% and 28.4%) were different slightly. The more the fatty oil in Semen Euphorbiae Pulveratun, the earlier and the more serious appearance of the ill symptom. ConclusionThe fatty oil in Semen Euphorbiae is the main toxicity contents and the chief toxicity symptom of Semen Euphorbiae is diarrhoea and back bowing. A new standard for the safety content range of fatty oil of 18.0% ~ 22.0% is proposed for Semen Euphorbiae Pulveratun on the basis of the results given above and actual situation of frostlike powder processing of Semen Euphorbiae.

Key words: Semen Euphorbiae, Semen Euphorbiae Pulveratun, acute toxicity, the content range of fatty oil

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