中国药物警戒 ›› 2018, Vol. 15 ›› Issue (7): 389-392.

• 基础与临床研究 • 上一篇    下一篇

逍遥丸对雷公藤多苷致大鼠排卵障碍模型卵巢氧化损伤和颗粒细胞凋亡的影响

周楠1, 杨宏敏2, 马惠荣3, 回丽敏1, 刘振兴1, 杜惠兰3,*   

  1. 1邢台市人民医院,河北 邢台 054031;
    2河北医科大学,河北 石家庄 050031;
    3河北中医学院,河北 石家庄 050200
  • 收稿日期:2018-08-08 修回日期:2018-08-08 出版日期:2018-07-20 发布日期:2018-08-08
  • 通讯作者: *杜惠兰,女,博士,教授·博导,中医妇科与生殖内分泌临床。E-mail:duhuilan@163.com
  • 作者简介:周楠,女,博士,主治医师,妇科临床。
  • 基金资助:
    国家自然科学基金（30772818）：补肾法和疏肝法对生殖功能影响特征的比较研究

Effect of Xiaoyao Pill on Ovarian Oxidative Damage and Apoptosis in Glycosides of Tripterygium Wilfordii- induced Ovulation Obstacles Rats

ZHOU Nan1, YANG Hongmin2, MA Huirong3, HUI Linmin1, LIU Zhenxing1, DUI Huilan3,*   

  1. 1Xingtai People's Hospital, Hebei Xingtai 054031, China;
    2Hebei Medical University, Hebei Shijiazhuang 050031, China;
    3Hebei University of Chinese Medicine, Hebei Shijiazhuang 050200, China
  • Received:2018-08-08 Revised:2018-08-08 Online:2018-07-20 Published:2018-08-08

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摘要： 目的 观察逍遥丸对雷公藤多苷致排卵障碍模型大鼠卵巢氧化损伤和颗粒细胞凋亡的影响。方法 建立雷公藤多苷致排卵障碍大鼠模型,136只Wistar大鼠随机分为空白对照组、模型组、逍遥丸高剂量组和低剂量组,每组34只。比色法测定血清和卵巢组织中Tbil、Dbil和T-AOC的含量,硫代巴比妥法测定MDA水平;末端脱氧核糖核苷酸转移酶介导的缺口末端标记（terminal deoxynucleotidyl transferase-mediated nick-end labeling,TUNEL）法检测卵巢颗粒细胞的凋亡水平。结果 与空白对照组比较,模型组大鼠血清及卵巢组织中Tbil、Dbil和T-AOC水平明显降低（均P <0.01）,MDA明显升高（均P <0.01）,卵巢颗粒细胞的凋亡率明显增加（P <0.01）。与模型组比较,逍遥丸（54、27 g·kg-1）明显提高大鼠血清和卵巢组织中Tbil、Dbil和T-AOC水平（均P <0.05）,降低卵巢组织中MDA水平（均P <0.05）及颗粒细胞的凋亡（P <0.01）,其中逍遥丸高剂量组药效最佳。结论 逍遥丸通过改善雷公藤多苷致排卵障碍模型大鼠的卵巢氧化损伤和颗粒细胞的凋亡,从而治疗排卵障碍性相关疾病。

关键词: 逍遥丸, 雷公藤多苷致大鼠排卵障碍模型, 氧化损伤, 细胞凋亡, 卵巢

Abstract: Objective To observe the effects of Xiaoyao pill (XYP) on ovarian oxidative damage and apoptosis in Glycosides of Tripterygium Wilfordii (GTW)-induced ovulation obstacles rats. Methods The rat model of ovulation caused by GTW was successfully established. One hundred and thirty-six rats were randomly divided into control group, model group, XYP high-dose group and XYP low-dose group, with 34 rats in each group. Serum and ovarian tissues levels of Tbil, Dbil and T-AOC were detected by colorimetry, and the levels of MDA were detected by penthiobarbital. Apoptosis of ovarian granulosa cells was observed by TUNEL. Results Compared with control group, serum and ovarian tissues MDA level increased(P <0.01), while Tbil, Dbil and T-AOC levels decreased in the model group (all P <0.01), the apoptosis of ovarian granulosa cells was significantly increased in model group (P <0.01). After treatment with XYP, the levels of serum and ovarian tissues Tbil and Dbil were increased (all P <0.05), while the levels of ovarian tissues MDA in those groups were lower than model group (all P <0.05). Moreover, the apoptosis of ovarian granulosa cells was significantly lower than those in model group (all P <0.01). XYP high-dose group shows the best effect. Conclusion XYP could improve follicular development and ovulation obstacles by regulating ovarian oxidative damage and apoptosis.

Key words: Xiaoyao pill, GTW-induced ovulation obstacles rats, oxidative damage, apoptosis, ovary

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