高压氧对大鼠体内格列齐特药动学的影响

中国药物警戒 ›› 2016, Vol. 13 ›› Issue (12): 720-723.

高压氧对大鼠体内格列齐特药动学的影响

曹秀琴¹,宋丹丹¹,沈佳伟¹, 周玮丽¹,施红¹,褚菲菲¹, 宋金波^2,*

1 上海市闵行区吴泾医院药剂科,上海 200241;
2 复旦大学附属上海市第五人民医院,上海 200040

收稿日期:2017-01-13 修回日期:2017-01-13 出版日期:2016-12-20 发布日期:2017-01-13
通讯作者: 宋金波,男,本科,副主任药师,临床药学与医院管理。E-mail：songjinbo168@163.com
作者简介:曹秀琴,女,本科,副主任药师,临床药学与医院药事管理。
基金资助:
上海市卫生和计划生育委员会科研基金资助项目（20134442）

Effects of Hyperbaric Oxygen on the Pharmacokinetics of Gliclazide in Rats

CAO Xiu-qin¹, SONG Dan-dan¹, SHEN Jia-wei¹, ZHOU Wei-li¹, SHI Hong¹, CHU Fei-fei¹, SONG Jin-bo^{2, *}

1 Department of Pharmacy, Wujing Hospital of Shanghai Minhang District, Shanghai 200241, China;
2 Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China;

Received:2017-01-13 Revised:2017-01-13 Online:2016-12-20 Published:2017-01-13

摘要/Abstract

摘要： 目的研究高压氧（HBO）对格列齐特（GL）药代动力学的影响。方法将16只大鼠随机分为对照组和高压氧组,2组均按30 mg·kg^-1剂量单次灌服格列齐特溶液。高压氧组灌服后立即置于高压氧舱,稳压（0.202 MPa）吸纯氧20 min,升压、减压各5 min;对照组仅单次灌服格列齐特溶液。2组在灌胃后0.67、1.0、1.5、2.0、3.0、4.0、6.0、8.0、12.0、24.0 h采血500 µL,采用超高效液相色谱-质谱联用法测定血浆中GL浓度,计算药代动力学参数。结果高压氧组峰浓度（C_max）、达峰时间（t_max）、血浆半衰期（t_1/2）、平均血药浓度一时间曲线下面积（AUC _0-∞）及消除速率常数（ke）分别为：119.81±25.19 µg·mL^-1、2.69±0.80 h、3.54±2.30 h、731.96±210.15 µg·mL^-1·h、0.26±0.13;对照组分别为：112.43±26.96 µg·mL^-1、2.75±1.00 h、2.55±1.10 h、653.60±184.20 µg·mL^-1·h、0.31±0.10。结论高压氧预处理对大鼠体内格列齐特药代动力学参数的影响无统计学意义。

关键词: 高压氧, 格列齐特, 药代动力学, 超高效液相色谱-质谱联用法

Abstract: Objective To investigate the effects of hyperbaric oxygen (HBO) on the pharmacokinetics of gliclazide (GL). Methods 16 rats were randomly divided into 2 groups, the HBO group and the control group. The 2 groups of animals received gavage of GL with a single-dose of 30 mg·kg^-1. The animals in the HBO group were immediately put into the hyperbaric oxygen chamber and maintained at a pressure of 0.20 MPa for 20 min. The time used for compression and decompression was both 5 min. The animals in the control group were just given a single gavage of GL. The blood samples from the rats were taken at 0.67, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 8.0, 12.0, 24.0 h after drug administration. Concentrations of GL in serum were monitored with UPLC-MS/MS, and pharmacokinetic parameters were calculated accordingly. Results The main pharmacokinetic parameters of the HBO group were as follows: C_max 119.81±25.19 µg·mL^-1, t_max 2.69±0.80 h, t_1/2 3.54±2.30 h, AUC 731.96±210.15 µg·mL^-1·h, ke 0.26±0.13, while the corresponding results of the control group were C_max 112.43±26.96 µg·mL^-1, t_max 2.75±1.00 h, t_1/2 2.55±1.10 h, AUC 653.60±184.20 µg·mL^-1·h, ke 0.31±0.10. Conclusion Effects of hyperbaric oxygen on the pharmacokinetics of gliclazide in rats was not significant.

Key words: hyperbaric oxygen, gliclazide, pharmacokinetics, UPLC-MS/MS

中图分类号:

R969.1

曹秀琴,宋丹丹,沈佳伟, 周玮丽,施红,褚菲菲, 宋金波. 高压氧对大鼠体内格列齐特药动学的影响[J]. 中国药物警戒, 2016, 13(12): 720-723.

CAO Xiu-qin, SONG Dan-dan, SHEN Jia-wei, ZHOU Wei-li, SHI Hong, CHU Fei-fei, SONG Jin-bo. Effects of Hyperbaric Oxygen on the Pharmacokinetics of Gliclazide in Rats[J]. Chinese Journal of Pharmacovigilance, 2016, 13(12): 720-723.

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