中国药物警戒 ›› 2010, Vol. 7 ›› Issue (4): 199-201.

• 基础研究 • 上一篇    下一篇

白花丹参对过氧化氢致人脐静脉血管内皮细胞损伤的保护作用

于长凯1, 张晓燕1, 高允生2*   

  1. 1江苏联合职业技术学院连云港中医药分院,江苏 连云港 222006;
    2泰山医学院,山东 泰安 271016
  • 收稿日期:2016-02-19 修回日期:2016-02-19 出版日期:2010-04-08 发布日期:2016-02-19
  • 通讯作者: 高允生,男,硕士生导师,中药药理与临床药理的研究。E-mail:ysgao@tsmc.edu.cn
  • 作者简介:于长凯,男,主治医师,药理学教学与科研。
  • 基金资助:
    山东省教育厅科研基金资助项目（J04E07）

Effect of Salvia Miltorrhiza bge.f.alba on Protecion of Vascular Endothelial Cells

YU Chang-kai1, ZHANG Xiao-yan1, GAO Yun-sheng2,*   

  1. 1Jiangsu Union Technical Institute Lianyungang Branch Institue, Jiangsu Lianyungang, 222006 China;
    2Taishan Medical University, Shandong Taian, 271016 China
  • Received:2016-02-19 Revised:2016-02-19 Online:2010-04-08 Published:2016-02-19

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摘要： 目的 研究白花丹参对过氧化氢（H2O2）所致人脐静脉血管内皮细胞（HUVEC）损失的保护作用。方法 应用酶消化灌注法培养人脐静脉血管内皮细胞,采用形态学观察和Ⅷ因子抗体免疫荧光检测法进行鉴定;取对数生长期的细胞分组进行干预,应用形态学观察法和MTT法检测各组血管内皮细胞的活性。结果 H2O2损伤模型组细胞损伤明显,经白花丹参高、中、低各剂量组的干预,受损情况均有较明显地改善,使细胞活性（OD值）增高。结论 白花丹参对H2O2所致的HUVEC损伤具有保护作用。

关键词: 白花丹参, 人脐静脉血管内皮细胞, 过氧化氢

Abstract: Objective To study the effect of Salvia miltiorrhiza bge.f.alba(SMA) on a model of injured human umbilical vascular endothelial cell(HUVEC) in vitro by hydrogen peroxide(H2O2). Methods In vitro perfusion and enzyme diges-tion method is used to culture HUVEC, and the cells are identified by morphological observation and Ⅷ immunoreactivity. Select the cells in the exponential phase of growth and treated, the cells viability is measured by morphological observation and the MTT assay. Results HUVEC are damaged obviously in H2O2 damage group, while in groups with SMA, condition of HUVEC is improved obviously, and the activity(OD value) of the cells is significantly increased. Conclusion SMA can protect HUVEC from damage by H2O2.

Key words: salvia miltiorrhiza bge.f.alba, human umbilical vascular endothelial cell(HUVEC), hydrogen peroxide(H2O2)

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