中国药物警戒 ›› 2015, Vol. 12 ›› Issue (2): 76-78.

• 基于毒-效-证关联评价的山豆根毒性研究专栏 • 上一篇    下一篇

山豆根水提组分对咽喉实热证小鼠急性毒性实验研究

赵庆华1，2,谢元璋3,李素君4,杨倩5,孙蓉1*   

  1. 1山东省中医药研究院，山东 济南 250014;
    2山东中医药大学，山东 济南250355;
    3烟台荣昌制药股份有限公司，山东 烟台264006;
    4山东省食品药品监督管理局，山东 济南250013;
    5日照市中医医院，山东 日照276800
  • 收稿日期:2014-08-21 出版日期:2015-02-08 发布日期:2015-07-28
  • 通讯作者: 孙蓉，女，博士，研究员·博导，中药药理与毒理
  • 作者简介:赵庆华，女，硕士，中药药理与毒理研究。

Study on Acute Toxicity of Water Extract of Sophorae Tonkinensis Radix et Rhizome on Throat Excess-heat Syndrome Mice

ZHAO Qing-hua1,2，XIE Yuan-zhang3，LI Su-jun4，YANG Qian5，SUN Rong1*   

  1. 1Shandong Research Academy of TCM, Shandong Ji'nan 250014 China;
    2Shandong University of Traditional Chinese Medicine, Shandong Ji'nan 250355, China;
    3Yantai Rongchang Pharmaceutical Co. Ltd. , Shandong Yantai 264006, China;
    4Shandong Food and Drug Administration Ji'nan 250013, China;
    5Rizhao Hospital of TCM, Shandong Rizhao 276800, China
  • Received:2014-08-21 Online:2015-02-08 Published:2015-07-28

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摘要： 目的 进行山豆根水提组分对咽喉实热证小鼠的急性毒性研究，以阐释清热解毒类有毒中药“毒性-证候”相关性。方法 制备山豆根水提组分样品，建立小鼠咽喉实热证模型，采用经典的小鼠急性毒性实验方法 给模型小鼠灌胃山豆根水提组分进行急性毒性实验，连续观察14天，观察其急性毒性症状、体征，记录累积死亡率和小鼠体重变化，计算LD50 。结果 山豆根水提组分给咽喉实热证模型小鼠灌胃后小鼠出现烦躁、呼吸抑制等急毒症状、体征，LD50及其95%可信限为20.63（17.94~23.92）g·kg-1。结论 山豆根水提组分对咽喉实热证模型小鼠的急性毒性明显小于正常动物，毒性症状出现时间延后，毒性症状减轻，LD50值增大。

关键词: 山豆根, 水提组分, 咽喉实热证, 急性毒性

Abstract: Objective The acute toxicity of water extract of Sophorae tonkinensis Radix et Rhizome on mice was researched to determine the correlation of "toxicity-syndrome". Methods The sample water extract of Sophorae tonkinensis Radix et Rhizome was administrated to KM mice of the throat excess- heat syndrome models. The LD50 of Sophorae tonkinensis Radix et Rhizome was tested by the classical Methods of acute toxicity. The acute toxic symptoms were observed, the accumulated death number and the weight-changes of live mice were recorded. Results The LD50 and its 95% confidence limits of water extract of Sophorae tonkinensis Radix et Rhizome was 20.63(17.94～23.92)g·kg-1 in mice. Restlessness, breathlessness and convulsion were the main acute toxic symptoms. Conclusion The acute toxicity of water extract of Sophorae tonkinensis Radix et Rhizome on throat excess-heat syndrome mouse model in mice was significantly less than normal animals. The onset time of toxic symptoms was delayed and relieved, the LD50 value was increased.

Key words: Sophorae tonkinensise Radix et Rhizome, water extract, throat excess-heat, acute toxicity

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