中国药物警戒 ›› 2014, Vol. 11 ›› Issue (7): 385-389.

• 基础与临床研究 •    下一篇

复方一枝蒿颗粒对防治手足口病的体内外药效学研究

郭姗姗1, 马雪萍2, 潘昕1, 高英杰1, 崔晓兰1*   

  1. 1中国中医科学院中药研究所,北京 100700;
    2新疆维吾尔自治区药物研究所,新疆 乌鲁木齐 830004
  • 收稿日期:2016-02-03 修回日期:2016-02-03 出版日期:2014-07-08 发布日期:2016-03-02

The Pharmacodynamics Research on Preventive and Therapeutic Effects of Compound Yizhihao Granules on Hand-foot-mouth Disease

GUO Shan-shan1, MA Xue-ping2, PAN Xin1, GAO Ying-jie1, CUI Xiao-lan1, *   

  • Received:2016-02-03 Revised:2016-02-03 Online:2014-07-08 Published:2016-03-02

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摘要： 目的通过观察复方一枝蒿颗粒在体内外对肠道病毒的抑制作用,评价复方一枝蒿颗粒防治手足口病的药效作用。方法采用复方一枝蒿颗粒的3岁儿童临床2倍、等倍和1/2倍剂量分别对肠道病毒EV71 H株感染的Babl/c乳鼠手足口病动物模型进行预防性给药和治疗性给药,通过观察乳鼠感染的严重程度、死亡数,来计算动物死亡率、死亡保护率和生命延长率;采用细胞病变的CPE法观察复方一枝蒿颗粒对肠道病毒EV71 H株,BrCr株,柯萨奇病毒B3、B4、B5株的抑制作用;采用real time PCR法观察复方一枝蒿颗粒对肠道病毒EV71 BrCr株在RD细胞中增殖的抑制作用。结果复方一枝蒿颗粒对肠道病毒EV71 H株感染的乳鼠模型具有明显的治疗作用,其可降低感染程度和死亡率,具有明显的死亡保护和延长生命的作用;复方一枝蒿颗粒在无明显毒性的浓度下,对肠道病毒EV71 H株、BrCr株具有明显的抑制作用,其能明显抑制EV71 BrCr株在细胞内的增殖。结论复方一枝蒿颗粒在体内外对手足口病均具有较好的治疗作用,其作用机制可能与抑制肠道病毒在细胞内增殖有关。

关键词: 复方一枝蒿颗粒, 手足口病, EV71, Balb/c乳鼠, 病毒增殖

Abstract: Objective To evaluate the preventive and therapeutic effects of Compound yizhihao granules on hand-foot-mouth disease by observing the death protective effect of Compound yizhihao granules on suckling Balb/c mice infected by enterovirus 71 in vivo and the inhibition effect on enteric viruses in vitro. Methods Prophylactic admi-nistration and therapeutic administration of Compound yizhihao granules were applied to EV71 H strain infection suckling Balb/c mice at 2、1 and 1/2 times clinical equivalent dose of 3-year-old children respectively, we observed degree of infection, death number and calculated the death rates, death protection rates and life extension rates. In vitro, the effect of Compound yizhihao granules at four non-toxic dosages anti EV71 H strain, EV71 BrCr strain, CoxB3 strain, CoxB4 strain and CoxB5 strain was determined by CPE method on Vero cells. Furthermore virus proliferation in RD cells was determined by real time PCR. Results Compound yizhihao granules decreased degree of infection and death rates, simultaneously increased death protection rates and life extension rates significantly. Compound yizhihao granules showed significant therapeutic effects on suckling Balb/c mice infected by enterovirus 71 H strain. At no-toxic dosages, Compound yizhihao granules inhibited cytopathic effect induced by EV71 H strain, and EV71 BrCr strain visibly and virus proliferation of EV71 BrCr strain was inhibited by Compound yizhihao granules significantly. Conclusion Compound yizhihao granules showed notable therapeutic effects on hand-foot-mouth disease in vivo and in vitro, the mechanism maybe related to the inhibition of virus proliferation in cells.

Key words: Compound yizhihao granules, hand-foot-mouth disease, enterovirus 71, suckling Balb/c mice, virus proliferation

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