中国药物警戒 ›› 2012, Vol. 9 ›› Issue (1): 9-15.

• 香加皮毒性研究 • 上一篇    下一篇

香加皮不同组分大鼠长期毒性研究

黄伟1，张勇3，钱晓路2，鲍志烨2，孙蓉1，*   

  1. 1 山东省中医药研究院，山东济南250014；
    2 山东中医药大学，山东济南 250355；
    3 山东省中医院，山东济南250012
  • 收稿日期:2010-10-11 出版日期:2012-01-10 发布日期:2015-08-10
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）,山东省科技平台建设项目课题（2008GG2NS02021）

Experimental Study on Chronic Toxicity in Rats Caused by Different Components of Cortex Periplocae Radicis

HUANG Wei1, ZHANG Yong3, QIAN Xiao-lu2, BAO Zhi-ye2, SUN Rong1, *   

  1. 1 Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2 Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    3 Shandong Province Hospital of Traditional Chinese Medicine, Shandong Jinan 250012, China
  • Received:2010-10-11 Online:2012-01-10 Published:2015-08-10

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摘要： 目的观察连续给予香加皮不同组分导致大鼠慢性毒性的损伤表现、程度及可逆性。方法分别给140 只大鼠灌胃高、中、低剂量的香加皮水提组分和醇提组分样品，除观察一般状况外，检测血常规、血生化指标，剖杀大鼠，精密称取心、肝、脾、肺、肾脏等重要脏器，计算脏、体比值，进行常规病理检查。停药后，进行恢复期观察。结果连续20天给予不同剂量的香加皮水提组分和连续9 天给予不同剂量的醇提组分样品均可导致大鼠体重下降，饮食、饮水不佳，血ALT、AST、AKP、TPC 增高，ALB、CR 降低、A/G 比值降低，肝脏重量和肝体比值、肾脏重量和肾体比值增大，病理检查可见不同程度的肝脏、肾脏病理组织损伤;对血常规影响不明显;肝、肾毒性损伤程度与给药剂量呈现一定的剂量依赖相关性;经过恢复期观察，上述部分病变不可逆。结论香加皮水提组分给药20 天、醇提组分给药9 天造成的慢性毒性损伤部位以肝、肾损伤为主，且部分病变为不可逆性损伤。

关键词: 香加皮, 不同组分, 大鼠, 长期毒性

Abstract: Objective The obvious toxic symptoms and signs, the toxic damage extents and reversibility were observed in rats caused by different components of Radicis. MethodsThe 140 rats were divided into seven groups such as normal group、water extract and alcohol extract components of high, medium and low dose. The general including the bodyweight、diet and behavior were observed , the blood routine, the biochemical indicators, organ/bodyweight rate ,the important organs such as heart、liver、spleen、lung、kidney、brain and et al. and pathological changes were detected after 20 days water extract and 9 days alcohol extract components administrated, and then we continued to observe the recovery period after drug discontinuance. ResultsThe Wistar rat's weight、diet and drinking were decreased; the serum ALT、AST、AKP、TPC were increased; the ALB、CR and the rate of A/G were decreased; the liver weight and volume ratio、kidney weight and volume ratio were increased after given water extract and alcohol extract components from Radicis to Wistar rats by intragastric for 21 and 9 days. Pathological examination showed different degree of liver and kidney pathology tissue damage; There was little affect for routine blood; the damage of liver and kidney toxicity in rats show an obvious dosage relationship. and after the convalescence, the partial lesions were irreversible. ConclusionThe main chronic toxical damage is hepatic and kidney injury in rats caused by the 20 days water extract and 9 days alcohol extract components of Cortex Periplocae Radicis,and part of the lesions were irreversible.

Key words: Cortex Periplocae Radicis, different components, rats, chronic toxicity