中国药物警戒 ›› 2021, Vol. 18 ›› Issue (12): 1101-1105.
DOI: 10.19803/j.1672-8629.2021.12.01

• 中药治疗病毒性肺炎的药理及机制研究专栏 • 上一篇    下一篇

热毒宁吸入溶液对甲型流感病毒A/PR/8/H1N1感染所致小鼠病毒性肺炎的药理作用研究

孙静1, 赵荣华1, 时宇静1, 张广平1, 包蕾1, 耿子涵1, 宋玲1, 高云航1, 李晓璐2, 郭姗姗1,#, 崔晓兰1#   

  1. 1中国中医科学院中药研究所,北京100700;
    2卫生部北京医院心内科,北京 100730
  • 收稿日期:2020-11-30 出版日期:2021-12-15 发布日期:2021-12-16
  • 通讯作者: *郭姗姗,女,副研究员,博士·硕导,中药抗病毒及抗感染药理。E-mail: ssguo@icmm.ac.cn. #为共同通信作者。
  • 作者简介:孙静,女,博士,中药抗病毒及抗感染药理。
  • 基金资助:
    重大新药创制国家科技重大专项（2017ZX09201002-006）; 中国中医科学院中医药防治流感技术体系（ZZ13-035-09）

Pharmacological Effects of Reduning Inhalation Solution on Viral Pneumonia in Mice Infected by Influenza A/PR/8/H1N1

SUN Jing1, ZHAO Ronghua1, SHI Yujing1, ZHANG Guangping1, BAO Lei1, GENG Zihan1, SONG Ling1, GAO Yunhang1, LI Xiaolu2, GUO Shanshan1,*, CUI Xiaolan1#   

  1. 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2Department of Cardiology, Beijing Hospital, Beijing 100730, China
  • Received:2020-11-30 Online:2021-12-15 Published:2021-12-16

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摘要： 目的 探讨热毒宁吸入溶液对甲型流感病毒A/PR/8/H1N1感染小鼠致肺炎模型的药理作用,为临床应用提供基础。方法 实验动物按体质量随机分为8组,分别为正常对照组、模型对照组、利巴韦林对照组、热毒宁注射给药对照组,热毒宁雾化吸入给药高剂量组0.23 g生药/kg（20 min）、中剂量组0.12 g生药/kg（10 min）、低剂量组0.06 g生药/kg（5 min）和极低剂量组0.03 g生药/kg（2.5 min）。采用甲型流感病毒A/PR/8/H1N1感染小鼠致肺炎模型,通过检测肺指数、肺部病理变化及肺组织中病毒载量、炎症因子白介素-6（IL-6）和肿瘤坏死因子α（TNF-α）的含量等指标评价热毒宁吸入溶液治疗流感病毒性肺炎的药理作用。结果 热毒宁吸入溶液4个剂量组均可不同程度上降低小鼠肺指数、减轻肺组织病理损伤、降低病毒载量及减少IL-6、TNF-α的含量。结论 热毒宁吸入溶液雾化吸入给药对甲型流感病毒A/PR/8/H1N1感染所致小鼠病毒性肺炎具有显著治疗作用。

关键词: 甲型流感病毒A/PR/8/H1N1, 病毒性肺炎, 热毒宁, 吸入溶液, 病毒载量, 炎症因子

Abstract: Objective To explore the therapeutic effect of Reduning inhalation solution against the viral pneumonia model of mice infected with influenza A/PR/8/H1N1 so as to provide reference for clinical application.Methods Laboratory animals were randomly divided into eight groups according to their body weight: control group, model group, ribavirin group, Reduning injection group, and four Reduning aerosol inhalation groups including the 0.23 g crude drug/kg high-dose group (20 min), 0.12 g crude drug/kg middle-dose group (10 min) , 0.06 g crude drug/kg low-dose group (5 min) and 0.03 g crude drug/kg very-low-dose group (2.5 min) . A pneumonia model of mice was established via intranasal infection with influenza A/PR/8/H1N1. The effectiveness of Reduning inhalation solution was evaluated by detecting the lung index, lung index inhibition rate, pathological changes, viral load, as well as the production of IL-6 and TNF-α in lung tissue. Results All the four treatments with Reduning inhalation solution could reduce the lung index of mice, alleviate the pathological damage to lung tissue, and reduce the viral load and the content of IL-6 and TNF-α in lung tissue to different extents.Conclusion Aerosol inhalation of Reduning has a significant therapeutic effect against the pneumonia model infected with influenza A/PR/8/H1N1.

Key words: influenza A/PR/8/H1N1, virus-associated pneumonia, Reduning, inhalation solution, viral load, inflammatory cytokine

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