中国药物警戒 ›› 2021, Vol. 18 ›› Issue (8): 748-752.
DOI: 10.19803/j.1672-8629.2021.08.10

• 基础与临床研究 • 上一篇    下一篇

茯苓对温和慢性不可预知应激大鼠行为学及粪便菌群的影响

徐燕, 刘冬光, 宁停波, 姚景春, 张贵民, 程金来*   

  1. 鲁南制药集团股份有限公司,中药制药共性技术国家重点实验室,山东 临沂 273400
  • 收稿日期:2020-07-17 出版日期:2021-08-15 发布日期:2021-08-17
  • 通讯作者: *程金来,本科,工程师,中药新药研究与开发。E-mail：13562990266@126.com
  • 作者简介:徐燕,硕士,工程师,中药新药研发与安全性评价。
  • 基金资助:
    国家重点研发计划（2019YFC1711205,2019YFC1711200）; 2019山东省重点扶持区域引进急需紧缺人才计划鲁发改重大办（（2019）391号文）

Effects of Poria on Behavior and Intestinal Flora in Rats with Chronic Unpredictable Mild Stress

XU Yan, LIU Dongguang, NING Tingbo, YAO Jingchun, ZHANG Guimin, CHENG Jinlai   

  1. Lunan Pharmaceutical Group Co. Ltd., State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Linyi Shandong 273400, China
  • Received:2020-07-17 Online:2021-08-15 Published:2021-08-17

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摘要： 目的 研究茯苓对慢性不可预知温和应激抑郁模型大鼠行为学及粪便菌群的影响。方法 采用大鼠慢性不可预知温和应激模型（chronic unpredictable mild stress, CUMS）,进行糖水偏好实验评价茯苓给药大鼠的快感缺失情况,采用旷场实验进行大鼠行为学分析,采用16S rRNA基因测序方法进行粪便菌群物种差异分析。结果 与正常组比较,模型组大鼠体重及糖水消耗率明显下降,自主活动明显减弱,粪便菌群物种丰富度和物种多样性减少,门水平上的拟杆菌门和变形菌门水平升高,厚壁菌门水平下降,属水平上的乳酸菌属水平下降;大鼠给予茯苓微粉后,与模型组比较,茯苓微粉组大鼠体重及糖水消耗量显著提高（P<0.05）,自主活动显著增强（移动距离增加、进入中央次数增加、垂直次数增加）且差异极显著（P<0.01）,粪便菌群物种丰富度和物种多样性增加,门水平上的拟杆菌门和变形菌门水平降低,厚壁菌门水平上升;属水平上的乳酸菌水平上升。结论 茯苓微粉通过降低拟杆菌门和变形菌门的水平以及恢复厚壁菌门和乳酸菌属的水平来实现抗抑郁作用。

关键词: 茯苓, 抗抑郁, 慢性不可预见应激, 旷场实验, 粪便菌群

Abstract: Objective To study the effect of Poria on behavior and intestinal microflora of chronic unpredictable depression stress model rats. Methods A chronic unpredictable mild stress (CUMS) model of rats was used to conduct a sugar water preference experiment to evaluate the lack of pleasure in rats administered with Poria cocos, and an open field experiment was used to analyze the behavior of rats. 16 rRNA gene sequencing method for intestinal flora species difference analysis. Results Compared with the normal group, the model group's body weight and sugar water consumption rate decreased significantly, autonomous activity was significantly weakened, the intestinal flora species richness and species diversity decreased, and the levels of Bacteroides and Proteobacteria at the portal level increased High, the level of thick-walled phylum decreased, and the level of genus lactic acid bacteria decreased; after the rats were given Poria powder, compared with the model group, the body weight and sugar water consumption of Poria powder group increased significantly (P<0.05), autonomous Significantly increased activity (increased moving distance, increased number of entry into the center, increased number of verticals) and the difference was extremely significant (P<0.01), intestinal flora species richness and species diversity increased, Bacteroidetes and Proteobacteria at the portal level The level of phylum decreased, the level of thick-walled phylum increased; the level of lactic acid bacteria at the genus level increased. Conclusion Poria cocos powder can achieve anti-depressive effect by reducing the level of Bacteroidetes and Proteobacteria, and restoring the level of Pachyphyta and Lactobacillus.

Key words: Poria, antidepressant, chronic unpredictable stress, open field experiment, intestinal flora

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